-transparent.png){kind=logo}
Because dipeptidylpeptidase IV (DPP IV) is present in vivo on glomerular visceral epithelial cells and possesses immunogenic properties, as shown by the capability of anti-DPP IV antibody to induce the Heymann model of glomerulonephritis, we studied the expression and regulation of DPP IV in cultured human glomerular visceral epithelial cells. DPP IV is an ectoenzyme, as indicated by the rapid detection of the product of the reaction in the incubation medium of intact cells and the staining of paraformaldehyde-fixed cells in the presence of a specific anti-DPP IV antibody. DPP IV activity was inhibited by diisopropylfluorophosphate and phenylmethyl sulphonylfluoride. Its optimum pH was alkaline (7.7-8) and it exhibited a Km value of 0.94 mM. DPP IV expression was induced in cells treated by interferon-gamma (IFN-gamma). The effect was significant after a 3-day treatment with 100 U/ml. It increased with time, reaching a plateau after 11 days, and was dose-dependent with a maximum at a concentration of 1000 U/ml. Staining of the cells with anti-DPP IV antibody was also increased after a 6-day treatment with 100 U/ml IFN-gamma. It was shown by Northern analysis that, after 24 hr of exposure to 500 U/ml of IFN-gamma, DPP IV mRNA transcript was stimulated. Transcriptional activation by IFN-gamma did not require new protein synthesis. Interleukin-1 (IL-1) and cyclic AMP had a small stimulatory effect, whereas dexamethasone and phorbol esters were inefficient. These results suggest that DPP IV of glomerular epithelial cells may be up-regulated by IFN-gamma from activated T lymphocytes in glomerular diseases and during lymphocyte-mediated graft rejection.
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
October 1993,
European journal of immunology,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
March 2003,
The Journal of biological chemistry,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
January 1991,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
September 2005,
The Prostate,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
September 1994,
Annals of surgical oncology,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
January 2012,
Biological & pharmaceutical bulletin,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
November 1994,
The American journal of physiology,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
October 1992,
Biochemistry international,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
August 1989,
The British journal of ophthalmology,
V Stefanovic, and
N Ardaillou, and
P Vlahovic, and
S Placier, and
P Ronco, and
R Ardaillou
September 1993,
Clinical and experimental immunology,
