The distributions of the cholinergic system and catecholaminergic system in the normal human brain were analysed quantitatively by a microphotometry system. Consecutive coronal sections were obtained from the anterior area of the left hemisphere and were stained alternately with fluorescent immunohistochemical staining for choline acetyltransferase or tyrosine hydroxylase. Each stained section was divided into approximately 120,000 areas and the fluorescence intensity in each area was measured by a fluorescence microphotometry system which is a measuring microscope for distribution of fluorescence intensity in the tissue slice. Nonspecific autofluorescence was distributed in myelinated nerve fiber throughout the entire area, which was subtracted from the fluorescence intensity value in each measuring area. The obtained immunohistochemical fluorescence intensities of choline acetyltransferase and tyrosine hydroxylase were classified into eight ranks and were indicated by color graphics. Also, the intensity values of actual immunohistochemical fluorescence in the various brain regions were presented. The choline acetyltransferase and tyrosine hydroxylase concentrations varied greatly depending on the brain region. Relatively high levels of choline acetyltransferase and tyrosine hydroxylase were distributed in the putamen, caudate nucleus, claustrum, insula and some cortical regions. The immunohistochemical level of tyrosine hydroxylase was lower than that of choline acetyltransferase in a few brain regions such as the globus pallidus and amygdala. High levels of choline acetyltransferase and tyrosine hydroxylase were localized in the one area of the basal ganglia which developed from the telencephalic area, whereas middle levels of these were distributed in another, part of which developed from the diencephalic area.(ABSTRACT TRUNCATED AT 250 WORDS)