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The gene (pepN) encoding an aminopeptidase N (PepN) has been cloned from Streptomyces lividans. This was done using either leucine-beta-naphthylamide or arginine-beta-naphthylamide in a liquid overlayer on colonies growing on agar medium to screen for overproduction of the ability to hydrolyse the substrates. The nucleotide sequence of pepN was determined and shown to encode a 95-kDa protein, which displayed significant homology to PepN proteins from other organisms. Analysis of the overproduced proteinase confirmed that this protein was located intracellularly as a monomeric active species. PepN is a metallo-exopeptidase cleaving next to Leu, Arg and Lys in peptide-bond-containing substrates.
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
August 1995,
Applied and environmental microbiology,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
January 1986,
Gene,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
January 1990,
Gene,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
July 1992,
FEBS letters,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
January 1993,
Gene,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
October 1994,
FEBS letters,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
March 1989,
Journal of biochemistry,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
February 1994,
Molecular & general genetics : MGG,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
January 1989,
Doklady Akademii nauk SSSR,
M J Butler, and
J S Aphale, and
C Binnie, and
M A DiZonno, and
P Krygsman, and
G A Soltes, and
E Walczyk, and
L T Malek
January 1993,
Gene,
