BIOMAT Database Logo BIOMAT Database Logo

Regulatory role of intraislet somatostatin on insulin secretion in the isolated perfused human pancreas. 1994

R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Department of Surgery, VAMC-West Los Angeles.

This study was undertaken to determine whether intraislet somatostatin inhibits insulin secretion in the human islet. A high-affinity monoclonal somatostatin antibody was used to immunoneutralize somatostatin in the isolated, perfused human pancreas. Single pass perfusion was performed in pancreata obtained from cadaveric organ donors using a modified Krebs medium with either 3.9 or 12.9 mM glucose. Sequential test periods separated by basal periods were performed with either somatostatin-14 (SS-14), somatostatin monoclonal antibody (CURE.S6), or a combined infusion. Infusion of SS-14 resulted in inhibition of insulin secretion under both low glucose (delta X = -712 +/- 212 pM) (p < 0.05) and high glucose (delta X = -21,913 +/- 10,003 pM) (p = 0.06) conditions. Immunoneutralization of intraislet somatostatin with CURE.S6 resulted in a significant increase in insulin secretion under both low glucose (454 +/- 162 pM) (p < 0.05) and high glucose (2,177 +/- 829 pM) (p < 0.05) conditions. Combined infusion of SS-14 and CURE.S6 resulted in a reversal of the inhibitory effect of exogenous SS-14. The data suggest that intraislet somatostatin has an inhibitory role in the regulation of insulin secretion in the human islet.

UI MeSH Term Description Entries
D007263 Infusions, Parenteral The administration of liquid medication, nutrient, or other fluid through some other route than the alimentary canal, usually over minutes or hours, either by gravity flow or often by infusion pumping. Intra-Abdominal Infusions,Intraperitoneal Infusions,Parenteral Infusions,Peritoneal Infusions,Infusion, Intra-Abdominal,Infusion, Intraperitoneal,Infusion, Parenteral,Infusion, Peritoneal,Infusions, Intra-Abdominal,Infusions, Intraperitoneal,Infusions, Peritoneal,Intra Abdominal Infusions,Intra-Abdominal Infusion,Intraperitoneal Infusion,Parenteral Infusion,Peritoneal Infusion
D007328 Insulin A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1). Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D007515 Islets of Langerhans Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN. Islands of Langerhans,Islet Cells,Nesidioblasts,Pancreas, Endocrine,Pancreatic Islets,Cell, Islet,Cells, Islet,Endocrine Pancreas,Islet Cell,Islet, Pancreatic,Islets, Pancreatic,Langerhans Islands,Langerhans Islets,Nesidioblast,Pancreatic Islet
D008297 Male Males
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D005260 Female Females
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000078790 Insulin Secretion Production and release of insulin from PANCREATIC BETA CELLS that primarily occurs in response to elevated BLOOD GLUCOSE levels. Secretion, Insulin

Related Publications

R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
The effect of intraislet somatostatin immunoneutralization on insulin secretion in the isolated perfused rat pancreas.
January 2000, International journal of surgical investigation,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Differential inhibition of insulin and islet amyloid polypeptide secretion by intraislet somatostatin in the isolated perfused human pancreas.
November 1999, Pancreas,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Intraislet somatostatin inhibits insulin (via a subtype-2 somatostatin receptor) but not islet amyloid polypeptide secretion in the isolated perfused human pancreas.
January 1997, Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Activation of somatostatin receptor subtype 2 inhibits insulin secretion in the isolated perfused human pancreas.
November 2003, Pancreas,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Somatostatin, insulin, and glucagon secretion from isolated perfused pancreas of obese rats.
August 1981, The American journal of physiology,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Splanchnic neural regulation of somatostatin secretion in the isolated perfused human pancreas.
March 1994, Annals of surgery,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Somatostatin release from isolated perfused rat pancreas. Possible role of endogenous somatostatin on insulin release.
June 1979, Diabetes,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Effect of insulin on glucose- and arginine-stimulated somatostatin secretion from the isolated perfused rat pancreas.
July 1981, Endocrinology,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Immunoneutralization of somatostatin, insulin, and glucagon causes alterations in islet cell secretion in the isolated perfused human pancreas.
October 2001, Pancreas,
R Kleinman, and G Ohning, and H Wong, and P Watt, and J Walsh, and F C Brunicardi
Insulin, glucagon, and somatostatin secretion from isolated perfused rat and chicken pancreas-duodenum.
February 1980, The American journal of physiology,
Copied contents to your clipboard!