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A synthetic gene encoding the peptide sequence for the low molecular weight (M(r) approximately 9600 Da) high-potential iron protein (HiPIP) from the photosynthetic bacterium Chromatium vinosum has been constructed by shotgun ligation of twelve complimentary oligonucleotides varying in size from 42-mers to 48-mers. After cloning the gene into a pET-21d(+) vector, expression of holoprotein in yields of 35 mg/liter of culture was obtained following induction with isopropyl-beta-D-thiogalactoside (IPTG). The recombinant protein was characterized by electronic absorption, 1H NMR, electrochemistry, N-terminal sequencing and amino acid analysis. This is the first example of the expression of a high potential ferredoxin containing a fully constituted [Fe4S4] cluster.
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
May 1997,
Biochimica et biophysica acta,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
February 1992,
Biochemistry,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
January 1976,
Biochemistry,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
October 1975,
Biochimica et biophysica acta,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
April 1995,
Journal of protein chemistry,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
June 1990,
Biochemistry,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
January 1995,
Biochemistry,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
October 1977,
Biophysical journal,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
April 1993,
Biochimica et biophysica acta,
A Agarwal, and
J Tan, and
M Eren, and
A Tevelev, and
S M Lui, and
J A Cowan
July 1996,
Biochimica et biophysica acta,
