Biomaterial Database

Rapid analysis of somatostatin in pharmaceutical preparations by HPLC with a micropellicular reversed-phase column. 1994

V Brizzi, and D Corradini

Dipartimento Farmaco Chimico Tecnologico, Università di Siena, Italy.

A rapid high-performance liquid chromatography method for the analysis of somatostatin in pharmaceutical preparations is described. A commercially available column packed with 2 microns spherical non-porous silica-based reversed-phase sorbent is used, along with a mobile phase consisting of acetonitrile and aqueous phosphoric acid, adjusted to pH 2.8 with sodium hydroxide. The effect of the organic modifier content and column temperature on the retention behaviour of somatostatin is reported. The method is found to be highly selective and specific, as indicated by the baseline separation of a mixture containing somatostatin and two analogue peptides, which differ from the analyte for one and two amino acids, respectively. Down to 10 ng of somatostatin can be detected and the detector response is linear over the concentration range from 4.14 to 20.75 micrograms ml-1. The application of this method to two commercial pharmaceutical formulations of somatostatin is found to give a mean percentage recovery from each of the two commercial samples, subjected to multiple injection analysis (n = 5), of 100.9% with a RSD of 0.92%, and 102.6% with a RSD of 1.56%, respectively.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D013004	Somatostatin	A 14-amino acid peptide named for its ability to inhibit pituitary GROWTH HORMONE release, also called somatotropin release-inhibiting factor. It is expressed in the central and peripheral nervous systems, the gut, and other organs. SRIF can also inhibit the release of THYROID-STIMULATING HORMONE; PROLACTIN; INSULIN; and GLUCAGON besides acting as a neurotransmitter and neuromodulator. In a number of species including humans, there is an additional form of somatostatin, SRIF-28 with a 14-amino acid extension at the N-terminal.	Cyclic Somatostatin,Somatostatin-14,Somatotropin Release-Inhibiting Hormone,SRIH-14,Somatofalk,Somatostatin, Cyclic,Somatotropin Release-Inhibiting Factor,Stilamin,Somatostatin 14,Somatotropin Release Inhibiting Factor,Somatotropin Release Inhibiting Hormone