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The raw material for ergocalciferol was tested for preparation of the "Ergocalciferol Reference Standard (Control 921)". Analytical data obtained were as follows: melting point, 118.1 degrees C; UV and infrared spectra, the same as those for the JP Ergocalciferol Reference Standard; specific absorbance, E1%1 cm = 457.5 (265 nm); thin-layer chromatography and high-performance liquid chromatography (HPLC), no impurities were detected; assay, 101.0% by HPLC. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 921).
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