Biomaterial Database

The agglutinating and immunofluorescent activities of antileptospiral antibodies of human sera and of immunoglobulins M and G. 1976

P Roch, and L Sramkova, and J Salak

Microagglutinating and immunofluorescent activities of sera of nine patients with clinically diagnosed leptospirosis were investigated. L. grippotyphosa was found in seven patients, L. sejroe in one and L. icterohaemorrhagiae in one patient. The agglutinating activities of IgM approximated the activities of the whole sera. In five patients the agglutinating activities were found in IgG; in four of them two to three months after the onset of the disease, in one patient a month after its onset. In one patient only the activity of IgG reached the activity of IgM after six months. Immunofluorescent activity was found in IgG in eight patients two to five weeks after the onset of the disease. In seven of these patients the activity of IgG reached or exceeded the activity of IgM three to ten weeks after the onset of the disease. IFAT was found to be poorly specific because of high heterologous titres. In 5 out of 7 patients with L. grippotyphosa, heterologous IFAT titres were reduced or eliminated by carrying out the reaction with isolated IgM and IgG. However, in patients with L. icterohaemorrhagiae and with L. sejroe heterologous IFAT activity persisted in both IgM and IgG. The study points out the possibility of differentiating an early stage of the infection from convalescence.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075	Immunoglobulin M	A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin.	Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007919	Leptospira	A genus of aerobic, helical spirochetes, some species of which are pathogenic, others free-living or saprophytic.
D007922	Leptospirosis	Infections with bacteria of the genus LEPTOSPIRA.	Cane-Cutter Fever,Canicola Fever,Leptospira Canicola Infection,Leptospira Infection,Leptospirosis Canicola,Mud Fever,Rice-Field Fever,Stuttgart Disease,Swineherd's Disease,Cane Cutter Fever,Fever, Canicola,Fever, Mud,Fever, Rice-Field,Fevers, Cane-Cutter,Infection, Leptospira,Infection, Leptospira Canicola,Infections, Leptospira,Infections, Leptospira Canicola,Leptospira Canicola Infections,Leptospira Infections,Leptospiroses,Leptospirosis Canicolas,Rice Field Fever,Swineherd's Diseases
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000371	Agglutination	The clumping together of suspended material resulting from the action of AGGLUTININS.