Abnormal incisor-tooth differentiation in transgenic mice expressing the muscle-specific desmin gene. 1993

M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
Laboratoire de Biologie-Odontologie, Institut Biomédical des Cordeliers, Université Paris VII, France.

Immunocytochemistry and electron microscopic observations on the incisor-tooth organ of transgenic mice expressing the muscle-specific desmin gene under the direction of the vimentin promoter, reveal that the expression of the hybrid transgene occurs both in mesenchymal cells and differentiating odontoblasts. The muscle-specific desmin, as estimated by fluorescence intensity, is more expressed in immature mesenchymal cells than in postmitotic differentiated odontoblasts. The expression of the transgene generates alteration of the odontoblast-intermediate filament network and interferes with the secretory activity of both odontoblasts and ameloblasts. Our results are consistent with the hypothesis that odontoblasts have inductive properties on the differentiation of ameloblasts and that intermediate filaments among other factors play the role of cell and tissue organizer.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007180 Incisor Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820) Incisors
D007382 Intermediate Filaments Cytoplasmic filaments intermediate in diameter (about 10 nanometers) between the microfilaments and the microtubules. They may be composed of any of a number of different proteins and form a ring around the cell nucleus. Tonofilaments,Neurofilaments,Filament, Intermediate,Filaments, Intermediate,Intermediate Filament,Neurofilament,Tonofilament
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009804 Odontoblasts The mesenchymal cells which line the DENTAL PULP CAVITY and produce DENTIN. They have a columnar morphology in the coronal pulp but are cuboidal in the root pulp, or when adjacent to tertiary dentin. Odontoblast
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003893 Desmin An intermediate filament protein found predominantly in smooth, skeletal, and cardiac muscle cells. Localized at the Z line. MW 50,000 to 55,000 is species dependent. Skeletin
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein

Related Publications

M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
March 1993, Development (Cambridge, England),
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
November 1996, European journal of cell biology,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
January 1993, Neuromuscular disorders : NMD,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
April 1988, The EMBO journal,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
June 2001, Proceedings of the National Academy of Sciences of the United States of America,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
October 1990, European journal of cell biology,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
July 1993, Differentiation; research in biological diversity,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
March 1989, The Journal of cell biology,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
April 1989, Proceedings of the National Academy of Sciences of the United States of America,
M V Berteretche, and I Dunia, and G Devilliers, and A van der Kemp, and F Pieper, and H Bloemendal, and E L Benedetti, and N Forest
April 2004, Circulation research,
Copied contents to your clipboard!