-transparent.png){kind=logo}
In vitro fertilized mouse eggs (C57BL/6N), followed by ultrarapid freezing were used for production of transgenic mice by microinjection of the chicken beta-actin promoter-driven the firefly luciferase cDNA (beta act-Luc). Following micromanipulation, the survival rates of the cryopreserved eggs and of the fresh in vitro fertilized eggs (control) were 70.8% (131/185) and 71.9% (159/221), respectively. After transferring them into oviducts of psudopregnant recipients on Day 1, 13.6% (17/125) of the cryopreserved eggs developed to live offspring and 14.1% (21/149) of fresh eggs did so. It was confirmed by Southern blotting analysis that each two transgenic mice were produced from the cryopreserved eggs (12%, 2/17) and the fresh eggs (10%, 2/21). All of transgenic mice produced from both eggs showed the expression of the luciferase gene. These results indicate that the in vitro fertilized eggs cryopreserved by ultrarapid freezing, can be, easily and conveniently, used for generation of transgenic mice.
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
December 1991,
Molecular reproduction and development,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
January 1997,
Biology of reproduction,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
January 1992,
Folia biologica,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
January 2008,
Methods in molecular biology (Clifton, N.J.),
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
January 1999,
Methods in molecular biology (Clifton, N.J.),
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
February 2001,
Molecular biotechnology,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
March 1974,
Poultry science,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
February 1982,
The Journal of experimental zoology,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
August 2004,
Theriogenology,
M Anzai, and
N Nakagata, and
K Matsumoto, and
T Ishikawa, and
Y Takahashi, and
K Miyata
June 1975,
Journal of reproduction and fertility,
