Clonal analysis and virulence of Australian isolates of Streptococcus suis type 2. 1994

C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
School of Veterinary Studies, Murdoch University, Western Australia.

Multilocus enzyme electrophoresis was used to divide 124 Australian isolates of Streptococcus suis type 2 into 17 electrophoretic types (ETs). Isolates in ET 1 were the most frequent cause of disease amongst Western Australian pigs, but isolates of ET 8 were more commonly associated with disease in other Australian states. Multiple isolates from 10 of 19 farms all belonged to the same ET, whilst isolates from the other farms belonged to between 2 and 4 different ETs. Some isolates could be differentiated further by DNA restriction endonuclease analysis, whilst others with the same restriction pattern were located in different, but closely-related ETs. Fourteen isolates were tested for their virulence in mice. Most caused disease if given in high numbers, but isolates in ET 1 were virulent at lower dose rates. This virulent clone also was distinguished by the fact that 80% of isolates produced extracellular factor (EF).

UI MeSH Term Description Entries
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings
D013290 Streptococcal Infections Infections with bacteria of the genus STREPTOCOCCUS. Group A Strep Infection,Group A Streptococcal Infection,Group A Streptococcal Infections,Group B Strep Infection,Group B Streptococcal Infection,Group B Streptococcal Infections,Infections, Streptococcal,Infection, Streptococcal,Streptococcal Infection
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013553 Swine Diseases Diseases of domestic swine and of the wild boar of the genus Sus. Disease, Swine,Diseases, Swine,Swine Disease
D014774 Virulence The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS. Pathogenicity
D014904 Western Australia A state in western Australia. Its capital is Perth. It was first visited by the Dutch in 1616 but the English took possession in 1791 and permanent colonization began in 1829. It was a penal settlement 1850-1888, became part of the colonial government in 1886, and was granted self government in 1890. (From Webster's New Geographical Dictionary, 1988, p1329)

Related Publications

C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
January 2020, Frontiers in microbiology,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
December 2015, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
January 1997, Advances in experimental medicine and biology,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
December 1999, Veterinary microbiology,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
December 2011, Emerging infectious diseases,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
December 1992, FEMS microbiology letters,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
January 2010, Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
November 2013, Transboundary and emerging diseases,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
February 2002, Wei sheng wu xue bao = Acta microbiologica Sinica,
C G Mwaniki, and I D Robertson, and D J Trott, and R F Atyeo, and B J Lee, and D J Hampson
September 2009, International microbiology : the official journal of the Spanish Society for Microbiology,
Copied contents to your clipboard!