The hourly variation of SCC in milk throughout the period between milkings was studied in 60 half udders of 30 Manchega ewes (10 each in first, second, and third parities) for 2 consecutive d. The interval between milkings was 12 h. The effects half udder, sampling time and day, interaction hour by day, and parity were statistically significant and explained 58.9, 11.9, 1.3, .6, and .3%, respectively, of total variance. The hourly repeatabilities of SCC and log SCC within each day (.83 and .86, respectively) were high. The SCC increased 70% in the 1st h postmilking and then gradually decreased until the next milking. The variations in SCC from 6 h postmilking were statistically significant but of less quantitative importance than those from the previous hours. The mean SCC of a.m. and p.m. milkings were very similar on both days (112 and 114 x 10(3) cells/ml). The mean SCC difference of milk between a.m. and p.m. milkings was 14%. This variation could be considered to be normal in the ewe for an interval of 12 h between milkings. These results suggest the standardization of time of sampling to avoid erroneous classifications of sheep based on SCC of milk.