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An enzyme immunometric assay of LTC4 named SPIE-IA is described. The assay involves different sequential steps: (1) immunocapture of LTC4 by monoclonal anti-LTC4 antibodies coated on 96-well microtiter plates; (2) cross-linking of LTC4 via its amino group to the wells using glutaraldehyde; (3) treatment with HCl; (4) measurement of linked LTC4 using the same monoclonal anti-LTC4 antibodies labeled with acetylcholinesterase. A minimal detectable concentration of 2 pg/ml after 60 min of enzymatic reaction was obtained. Cross-reactivity was less than 15% with LTD4 or LTE4. The coefficient of variation was less than 6% in the 20-1000 pg/ml range. Good correlation was observed between SPIE-IA and a competitive enzyme immunoassay for biological samples. The different sequential steps of the assay are investigated.
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
January 1995,
Advances in experimental medicine and biology,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
October 1996,
Journal of immunological methods,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
June 1993,
Journal of immunological methods,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
June 2017,
Cold Spring Harbor protocols,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
February 1995,
Analytical biochemistry,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
June 2017,
Cold Spring Harbor protocols,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
February 1990,
Clinical chemistry,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
December 1987,
Journal of pharmacological methods,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
November 1995,
Annals of clinical biochemistry,
H Volland, and
B Vulliez Le Normand, and
S Mamas, and
J Grassi, and
C Créminon, and
E Ezan, and
P Pradelles
January 1987,
Journal of food protection,
