Response of the meadow vole (Microtus pennsylvanicus) to experimental inoculation with Borrelia burgdorferi. 1994

G D Campbell, and I K Barker, and R P Johnson, and P E Shewen, and S A McEwen, and G A Surgeoner
Canadian Cooperative Wildlife Health Centre, Department of Pathology, Ontario Veterinary College, University of Guelph.

The response of the meadow vole (Microtus pennsylvanicus) to infection by experimental inoculation with Borrelia burgdorferi was evaluated. Forty-two adult voles were inoculated subcutaneously with 0.5 x 10(6) spirochetes. Sera taken during the 196 day trial were tested by indirect fluorescent antibody (IFA) assay for antibodies to B. burgdorferi. Tissues from animals which died during the trial, and from animals killed at 28, 112 and 196 days post-inoculation (DPI), respectively, were cultured in BSK-II medium for < or = 6 weeks. They also were examined histologically for lesions and the presence of spirochetes. All inoculated animals developed antibodies by 14 DPI and maintained titers > or = 1:10 for the duration of the trial. Spirochetes were isolated from ears, bladder, and spleen. Spirochetes also were identified by Bosma-Steiner silver stain or tissue IFA assay in sections of ears, bladder, kidney and heart. Infection as confirmed by re-isolation persisted for < or = 111 days. No lesions were identified in association with the presence of spirochetes. No increase in mortality was observed in inoculated animals compared with controls. Sensitivity of the IFA test at a cut-off titer of 1:10 was 100% from > or = 14 DPI, but at 1:20 reached a maximum of 97%. Specificity at 1:10 was 84% and at 1:20 was 97%. Use of antiserum to Microtus immunoglobulin (Ig) in a double-layered test provided no significant advantages over use of a commercial fluorescein-conjugated anti-mouse Ig in a single-layered IFA test.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008193 Lyme Disease An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut. Lyme Borreliosis,B. burgdorferi Infection,Borrelia burgdorferi Infection,Lyme Arthritis,Arthritis, Lyme,B. burgdorferi Infections,Borrelia burgdorferi Infections,Borreliosis, Lyme,Disease, Lyme
D001743 Urinary Bladder A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION. Bladder,Bladder Detrusor Muscle,Detrusor Urinae,Bladder Detrusor Muscles,Bladder, Urinary,Detrusor Muscle, Bladder,Detrusor Muscles, Bladder
D003411 Arvicolinae A subfamily of MURIDAE found nearly world-wide and consisting of about 20 genera. Voles, lemmings, and muskrats are members. Clethrionomys,Cricetidae,Dicrostonyx,Lemmings,Lemmus,Mice, Red-Backed,Microtinae,Microtus,Muskrats,Ondatra,Voles,Arvicolines,Microtines,Mouse, Red-Backed,Myodes,Ondatra zibethicus,Arvicoline,Lemming,Mice, Red Backed,Microtine,Mouse, Red Backed,Muskrat,Red-Backed Mice,Red-Backed Mouse,Vole
D004423 Ear The hearing and equilibrium system of the body. It consists of three parts: the EXTERNAL EAR, the MIDDLE EAR, and the INNER EAR. Sound waves are transmitted through this organ where vibration is transduced to nerve signals that pass through the ACOUSTIC NERVE to the CENTRAL NERVOUS SYSTEM. The inner ear also contains the vestibular organ that maintains equilibrium by transducing signals to the VESTIBULAR NERVE. Vestibulocochlear System,Vestibulocochlear Apparatus,Apparatus, Vestibulocochlear,Ears,System, Vestibulocochlear
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D012376 Rodent Diseases Diseases of rodents of the order RODENTIA. This term includes diseases of Sciuridae (squirrels), Geomyidae (gophers), Heteromyidae (pouched mice), Castoridae (beavers), Cricetidae (rats and mice), Muridae (Old World rats and mice), Erethizontidae (porcupines), and Caviidae (guinea pigs). Disease, Rodent,Diseases, Rodent,Rodent Disease
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity

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