Biomaterial Database

Scanning electron microscopic study on the effect of tunicamycin on Candida albicans. 1993

M N Vespa, and J C Lebecq, and N Simonetti

Istituto di Microbiologia, Facoltà di Farmacia, Università degli Studi di Roma La Sapienzia, Italy.

Scanning electron microscopy was used to study the effect of tunicamycin, an inhibitor of N-glycosylation, on the morphology of Candida albicans grown in minimal essential medium Earle's base and supplemented with 0.5% N-acetyl-D-glucosamine to stimulate germ tube induction. Addition of the drug prevented development of germ tubes, development of mycelia from germ tubes and resumption of germ tube growth after drug removal. Inhibited cells increased in size during treatment and subsequently became misshapen and shrivelled with evidence of cytoplasmic spillage.

UI	MeSH Term	Description	Entries
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D002176	Candida albicans	A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).	Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D014415	Tunicamycin	An N-acetylglycosamine containing antiviral antibiotic obtained from Streptomyces lysosuperificus. It is also active against some bacteria and fungi, because it inhibits the glucosylation of proteins. Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms.