Evaluation of the albicans IDR plate method for the rapid identification of Candida albicans. 1993

V Lipperheide, and L Andraka, and J Pontón, and G Quindós
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.

Albicans IDR is a ready-to-use medium containing a hexosaminic substrate that allows rapid and specific identification of Candida albicans. In this study, we have evaluated its performance using 330 pure cultures of yeast strains previously identified by conventional mycological methods. The test correctly identified all the Candida albicans strains tested (186 isolates), but there were 28 false-positive reactions after 48 h of culture incubation. The false-positive isolates corresponded to the species Candida tropicalis (13 strains), Trichosporon beigelii (12 strains), Cryptococcus neoformans (one strain), Candida lusitaniae (one strain) and Candida membranaefaciens (one strain). The sensitivity of the method was 100% and the specificity was 90.3% at 24 h and 86.6% at 48 h of incubation. Albicans IDR plate is a new, easy-to-perform and inexpensive method for C. albicans identification, and it could be used for direct isolation and identification of this species from clinical samples.

UI MeSH Term Description Entries
D002176 Candida albicans A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis). Candida albicans var. stellatoidea,Candida stellatoidea,Dematium albicans,Monilia albicans,Myceloblastanon albicans,Mycotorula albicans,Parasaccharomyces albicans,Procandida albicans,Procandida stellatoidea,Saccharomyces albicans,Syringospora albicans
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D016533 Mycological Typing Techniques Procedures for identifying types and strains of fungi. Biotyping, Mycological,Fungal Typing Techniques,Typing, Fungal,Fungal Typing Technics,Mycological Biotyping,Mycological Typing Technic,Mycological Typing Technics,Mycological Typing Technique,Technic, Mycological Typing,Technics, Mycological Typing,Technique, Mycological Typing,Techniques, Mycological Typing,Typing Technic, Mycological,Typing Technics, Mycological,Typing Technique, Mycological,Typing Techniques, Mycological,Fungal Typing,Fungal Typing Technic,Fungal Typing Technique,Technic, Fungal Typing,Technics, Fungal Typing,Technique, Fungal Typing,Techniques, Fungal Typing,Typing Technic, Fungal,Typing Technics, Fungal,Typing Technique, Fungal,Typing Techniques, Fungal

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