Biomaterial Database

Cell wall synthesis regulation in Saccharomyces cerevisiae. Effect of RNA and protein inhibition. 1976

M V Elorza, and C M Lostau, and J R Villanueva, and R Sentandreu

In this investigation the regulation of wall formation in Saccharomyces cerevisiae ts-136 (Hutchison, H.T., Hartwell, L.H. and McLaughlin, C.S. (1969) J. Bacteriol. 99, 807-814) was analyzed by following the inhibition of RNA and protein synthesis. Lomofungin, thiolutin and 8-hydroxyquinoline at the concentrations needed to inhibit RNA synthesis also produced inhibition of glucan and mannan synthetases. The synthesis of RNA was also blocked in S. cerevisiae ts-136 by incubation at the non-permissive temperature (37 degrees C). Mannan formation decreased steadily but glucan synthesis remained after 4 to 5 h. After a few minutes of blocking protein synthesis with cycloheximide mannan synthesis was also blocked whereas glucan formation was unaffected by the presence of the drug. These results suggest a high degree of stability for glucan synthetases. S. cerevisiae ts-136 after 2 h of incubation at the non-permissive temperature (37 degrees C) showed a preferential formation of wall materials (mannan and glucan) indicating that the RNA messengers which codify wall mannan peptides have a slower decay rate than those of the cytoplasmic proteins. The data presented indicate that the existence of stable glucan synthetases and RNA messengers of the wall mannan peptides of slow decay rate results in the continuous synthesis of glucans and mannoproteins of the yeast wall throughout the cell cycle.

UI	MeSH Term	Description	Entries
D008351	Mannans	Polysaccharides consisting of mannose units.	Mannan
D008565	Membrane Proteins	Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.	Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D010619	Phenazines
D011134	Polysaccharides	Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds.	Glycan,Glycans,Polysaccharide
D011760	Pyrrolidinones	A group of compounds that are derivatives of oxo-pyrrolidines. A member of this group is 2-oxo pyrrolidine, which is an intermediate in the manufacture of polyvinylpyrrolidone. (From Merck Index, 11th ed)	Pyrrolidinone,Pyrrolidone,Pyrrolidones
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D003513	Cycloheximide	Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.	Actidione,Cicloheximide
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006912	Hydroxyquinolines	The 8-hydroxy derivatives inhibit various enzymes and their halogenated derivatives, though neurotoxic, are used as topical anti-infective agents, among other uses.	Quinolinols,Chiniofon