BIOMAT Database Logo BIOMAT Database Logo

Enhancement by serotonin of GABA-mediated inhibitory synaptic currents in rat cerebellar Purkinje cells. 1994

H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.

The actions of serotonin (5-HT) on synaptic responses in Purkinje cells were examined using the whole-cell voltage-clamp recording from thin slices of the rat cerebellum. Focal stimulation within the molecular layer elicited synaptic responses: (1) inhibitory postsynaptic currents (IPSCs) that were completely blocked by bicuculline; (2) excitatory postsynaptic currents (EPSCs) that were antagonized by CNQX; and (3) combination of IPSC and EPSC. Application of 5-HT (3-30 microM) increased the amplitude of the stimulation-evoked IPSCs but did not significantly affect the EPSCs. The frequency and the mean amplitude of spontaneous IPSCs were increased in the presence of 5-HT. Neither the sensitivity to exogenously applied GABA nor the holding current of Purkinje cells were noticeably altered by 5-HT. The results indicate that 5-HT differentially enhances the GABA-mediated IPSCs in rat cerebellar Purkinje cells via a presynaptic mechanism. Serotonergic neurons thus appear to play a role in the motor control through selective presynaptic facilitation that modulates the output from the cerebellar cortex.

UI MeSH Term Description Entries
D011689 Purkinje Cells The output neurons of the cerebellar cortex. Purkinje Cell,Purkinje Neuron,Purkyne Cell,Cell, Purkinje,Cell, Purkyne,Cells, Purkinje,Cells, Purkyne,Neuron, Purkinje,Neurons, Purkinje,Purkinje Neurons,Purkyne Cells
D002531 Cerebellum The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills. Cerebella,Corpus Cerebelli,Parencephalon,Cerebellums,Parencephalons
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005680 gamma-Aminobutyric Acid The most common inhibitory neurotransmitter in the central nervous system. 4-Aminobutyric Acid,GABA,4-Aminobutanoic Acid,Aminalon,Aminalone,Gammalon,Lithium GABA,gamma-Aminobutyric Acid, Calcium Salt (2:1),gamma-Aminobutyric Acid, Hydrochloride,gamma-Aminobutyric Acid, Monolithium Salt,gamma-Aminobutyric Acid, Monosodium Salt,gamma-Aminobutyric Acid, Zinc Salt (2:1),4 Aminobutanoic Acid,4 Aminobutyric Acid,Acid, Hydrochloride gamma-Aminobutyric,GABA, Lithium,Hydrochloride gamma-Aminobutyric Acid,gamma Aminobutyric Acid,gamma Aminobutyric Acid, Hydrochloride,gamma Aminobutyric Acid, Monolithium Salt,gamma Aminobutyric Acid, Monosodium Salt
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001640 Bicuculline An isoquinoline alkaloid obtained from Dicentra cucullaria and other plants. It is a competitive antagonist for GABA-A receptors. 6-(5,6,7,8-Tetrahydro-6-methyl-1,3-dioxolo(4,5-g)isoquinolin-5-yl)furo(3,4-e)1,3-benzodioxol-8(6H)one
D012701 Serotonin A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator. 5-HT,5-Hydroxytryptamine,3-(2-Aminoethyl)-1H-indol-5-ol,Enteramine,Hippophaine,Hydroxytryptamine,5 Hydroxytryptamine
D013569 Synapses Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions. Synapse
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018750 6-Cyano-7-nitroquinoxaline-2,3-dione A potent excitatory amino acid antagonist with a preference for non-NMDA iontropic receptors. It is used primarily as a research tool. CNQX,1,4-dihydro-2,3-dihydroxy-7-nitro-6-quinoxalinecarbonitrile,6-Cyano-2,3-dihydroxy-7-nitroquinoxaline,FG-9065,6 Cyano 2,3 dihydroxy 7 nitroquinoxaline,6 Cyano 7 nitroquinoxaline 2,3 dione,FG 9065,FG9065

Related Publications

H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Inhibitory synaptic currents in rat cerebellar Purkinje cells: modulation by postsynaptic depolarization.
October 1992, The Journal of physiology,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Rapid modulation of inhibitory synaptic currents in cerebellar Purkinje cells by BDNF.
September 2005, Synapse (New York, N.Y.),
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Synaptic currents in cerebellar Purkinje cells.
April 1990, Proceedings of the National Academy of Sciences of the United States of America,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Beta-adrenergic enhancement of inhibitory synaptic activity in rat cerebellar stellate and Purkinje cells.
August 1993, The Journal of physiology,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Calcium entry increases the sensitivity of cerebellar Purkinje cells to applied GABA and decreases inhibitory synaptic currents.
April 1991, Neuron,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
GABA(A) receptor expression and inhibitory post-synaptic currents in cerebellar Purkinje cells in dystrophin-deficient mdx mice.
February 2008, Clinical and experimental pharmacology & physiology,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Spontaneous GABA-mediated synaptic currents in cerebellar granule cells in culture.
June 1995, Neuroreport,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Inhibitory synaptic currents in stellate cells of rat cerebellar slices.
August 1993, The Journal of physiology,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells.
July 2015, Nature communications,
H Mitoma, and T Kobayashi, and S Y Song, and S Konishi
Developmental enhancement of alpha2-adrenoceptor-mediated suppression of inhibitory synaptic transmission onto mouse cerebellar Purkinje cells.
September 2008, Neuroscience,
Copied contents to your clipboard!