Hatchery contamination can result in exposure of newly hatched chicks to salmonellae at a time when they are most susceptible to colonization of their intestinal tract. Eggshell fragments, external rinses, and intestinal tracts from day-of-hatch chicks were sampled for salmonellae contamination. Chicks from the same hatching trays were then put in isolators or floor pens and fed a pelleted corn-soybean ration for 1 wk before external rinses and ceca from each chick were sampled for salmonellae. About 17% of eggshell, 21% of chick rinses, and 5% of intestines sampled at Day 0 were positive for salmonellae. No differences were observed between broiler hatcheries, but significant differences were seen between replications within hatching cabinets. Results from this study suggest a correlation between hatchery-acquired salmonellae and the production of potential seeder birds. No differences between eggshell and chick rinse samples were found (correlation = .81); therefore, eggshells are recommended as the best sample to determine hatchery salmonellae contamination.