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To determine whether vitamin A is involved in pancreatic alpha cell function, we tested for (a) effects of vitamin A deficiency on glucagon release from perifused islets and perfused pancreases, and (b) the presence of cytosolic retinol-binding proteins (CRBP) and retinoic acid-binding proteins (CRABP), in the glucagon-secreting alpha cell line, ln-R1-G9. Arginine 19 mM plus glucose 2.8 mM-stimulated glucagon secretion was markedly impaired in islets and pancreases of vitamin A-deficient rats or rats that had at some time been cycled through vitamin A deficiency (ever A-def) despite repletion with retinoids for 2-4 weeks. Insulin secretion was impaired likewise. Repletion starting early in the development of vitamin A deficiency and for a longer period of time (18 or 60 days) did not restore glucagon secretion, but did normalize insulin secretion. CRBP and CRABP were present in ln-R1-G9 cells. We conclude that (a) vitamin A deficiency is associated with a defect in glucagon secretion; (b) The defect in secretion occurs early in the course of vitamin A deficiency; (c) The defect persists despite repletion; and (d) The requirement of vitamin A for secretion and the presence of CRBP and CRABP in glucagon-secreting cells support a physiologic role for vitamin A at the alpha cell level.
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
March 1986,
Diabetologia,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
October 1983,
Endocrinology,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
January 1987,
The Journal of clinical investigation,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
July 1980,
The American journal of clinical nutrition,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
January 1979,
Journal de physiologie,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
February 2006,
Experimental eye research,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
January 1984,
Mechanisms of ageing and development,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
February 1984,
Journal of oral pathology,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
April 1989,
International journal of pancreatology : official journal of the International Association of Pancreatology,
B S Chertow, and
H K Driscoll, and
W S Blaner, and
P Meda, and
M B Cordle, and
K A Matthews
September 1973,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
