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Electrophoretic and immunochemical studies of Micrurus snake venoms. 1994

A Alape-Girón, and B Lomonte, and B Gustafsson, and N J Da Silva, and M Thelestam
Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San Jose.

The electrophoretic mobilities of venom components from 15 Micrurus species were studied by polyacrylamide gel electrophoresis. The venoms showed species-specific protein patterns under native (PAGE) or denaturing (urea-PAGE) conditions. However, electrophoretic patterns obtained by SDS-PAGE under reducing conditions were similar. The proteins of all venoms had mol. wts either in the range of 45 to 75 kDa or lower than 14.5 kDa. PAGE and urea-PAGE of single extraction venom samples from 22 M. nigrocinctus nigrocinctus specimens revealed some proteins completely conserved, whereas others exhibited intraspecies variation. Based on ELISA cross-reactivity studies with 11 monoclonal antibodies against M. n. nigrocinctus venom, venoms from M. n. nigrocinctus, M. nigrocinctus mosquitensis, M. fulvius fulvius, M. dumerilii carnicauda and M. albicinctus were included in the same antigenic group, whereas M. frontalis frontalis and M. frontalis braziliensis venoms constituted a second group. Micrurus alleni and M. spixii spixii showed reactivity patterns similar to groups 1 and 2, respectively. Venoms from M. surinamensis surinamensis, M. corallinus, M. ibiboboca, M. hemiprichii ortoni, M. lemniscatus helleri and M. mipartitus had unique cross-reactivity patterns with monoclonal antibodies against M. n. nigrocinctus venom.

UI MeSH Term Description Entries
D007106 Immune Sera Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen. Antisera,Immune Serums,Sera, Immune,Serums, Immune
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D004546 Elapid Venoms Venoms from snakes of the family Elapidae, including cobras, kraits, mambas, coral, tiger, and Australian snakes. The venoms contain polypeptide toxins of various kinds, cytolytic, hemolytic, and neurotoxic factors, but fewer enzymes than viper or crotalid venoms. Many of the toxins have been characterized. Cobra Venoms,Elapidae Venom,Elapidae Venoms,Naja Venoms,Cobra Venom,Elapid Venom,Hydrophid Venom,Hydrophid Venoms,King Cobra Venom,Naja Venom,Ophiophagus hannah Venom,Sea Snake Venom,Sea Snake Venoms,Venom, Cobra,Venom, Elapid,Venom, Elapidae,Venom, Hydrophid,Venom, King Cobra,Venom, Naja,Venom, Ophiophagus hannah,Venom, Sea Snake,Venoms, Cobra,Venoms, Elapid,Venoms, Elapidae,Venoms, Hydrophid,Venoms, Naja,Venoms, Sea Snake
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000997 Antivenins Antisera used to counteract poisoning by animal VENOMS, especially SNAKE VENOMS. Anti-Venin,Anti-Venom,Antivenin,Antivenom,Anti-Venins,Anti-Venoms,Antivenoms,Anti Venin,Anti Venins,Anti Venom,Anti Venoms
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D017815 Elapidae A family of extremely venomous snakes which include coral snakes, cobras and sea snakes. They are widely distributed, occurring in the Southern United States, South America, Africa, Southern Asia, Australia, and the Pacific Islands. Like viperids (VIPERIDAE), they have fangs in the front part of the upper jaw which discharge venom that is neurotoxic. Acanthophiinae,Notechis,Pseudonaja,Cobra,Elapid Snakes,Elapids,Cobras,Elapid,Elapid Snake,Snake, Elapid

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