Biomaterial Database

Monoclonal antibody to a Thy-1 related/associated molecule identifies a subpopulation of immature mouse thymocytes. 1994

X F Csar, and J R Underwood, and L C Zanon, and M T Hearn

Department of Biochemistry, Monash University, Clayton, Vic., Australia.

In this study, NMT-1 mAbs have been shown to identify a thymocyte cell surface molecule (Immature Thymocyte Marker-1, ITM-1) expressed on 86.2 to 90.8% of CD4+CD8+CD3-/lo/int cells within the thymus of Balb/c, CBA, C57B1/6, 129 Rej, A/J and AKR mice. Similarly, immunoprecipitation analysis of cell membrane extracts from 125Iodine-labelled thymocytes, using NMT-1mAbs, identified 14.5 and 18.3 kDa molecules from all strains of mice examined. Comparison of these immunoprecipitation profiles with those produced by rat IgG2b anti-Thy-1 antibodies indicated that NMT-1 mAbs recognized a subspecies of the Thy-1 molecule. Unlike the Thy-1 molecule, the expression of ITM-1 molecules in Balb/c, CBA, C57B1/6, 129Rej, A/J and AKR strains of mice was restricted to the thymus. The ITM-1 molecule was not expressed on lymphoid cells within the spleen, lymph node, bone marrow, peritoneal cavity and peripheral blood in these strains. Phenotypic characterization associated the expression of ITM-1 molecule with the CD4+CD8+CD3-/lo/int thymocyte subpopulation indicating that NMT-1 mAbs recognize an epitope on a distinct developmentally-related Thy-1 glycoform. The expression of the ITM-1 molecule on differentiating thymocytes may prove useful in further subdividing immature thymic subpopulations, particularly those implicated in positive and negative selection.

UI	MeSH Term	Description	Entries
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015704	CD4 Antigens	55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. They are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. T4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.	Antigens, CD4,CD4 Molecule,CD4 Receptor,CD4 Receptors,Receptors, CD4,T4 Antigens, T-Cell,CD4 Antigen,Receptors, Surface CD4,Surface CD4 Receptor,Antigen, CD4,Antigens, T-Cell T4,CD4 Receptor, Surface,CD4 Receptors, Surface,Receptor, CD4,Surface CD4 Receptors,T-Cell T4 Antigens,T4 Antigens, T Cell
D016176	T-Lymphocyte Subsets	A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.	T-Cell Subset,T-Cell Subsets,T-Lymphocyte Subset,Subset, T-Cell,Subset, T-Lymphocyte,Subsets, T-Cell,Subsets, T-Lymphocyte,T Cell Subset,T Cell Subsets,T Lymphocyte Subset,T Lymphocyte Subsets
D016827	CD8 Antigens	Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. T8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.	Antigens, CD8,Leu-2 Antigens,T8 Antigens, T-Cell,CD8 Antigen,Antigen, CD8,Antigens, Leu-2,Antigens, T-Cell T8,Leu 2 Antigens,T-Cell T8 Antigens,T8 Antigens, T Cell
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018800	Thy-1 Antigens	A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.	Antigens, CD90,Antigens, Thy-1,CD90 Antigens,CD90 Antigen,Thy-1 Antigen,Thy-1.1 Antigen,Thy-1.2 Antigen,Antigen, CD90,Antigen, Thy-1,Antigen, Thy-1.1,Antigen, Thy-1.2,Antigens, Thy 1,Thy 1 Antigen,Thy 1 Antigens,Thy 1.1 Antigen,Thy 1.2 Antigen