-transparent.png){kind=logo}
We developed a sensitive enzyme-linked immunosorbent assay (ELISA) for detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL) in human serum. A monoclonal antibody against MDA-LDL (ML25) used in our method recognized not only MDA-LDL but also other MDA-modified proteins. However, MDA-LDL was able to be detected specifically by using a combination of ML25 and an antibody specific for apolipoprotein B (apo B) (AB16), which was conjugated with beta-galactosidase. Using this method, measurable amounts of MDA-LDL were detected in the sera of 40 healthy individuals. MDA-LDL was observed to be mainly distributed in the human LDL fraction separated by density gradient ultracentrifugation, while in each lipoprotein subfraction the largest amount of MDA-LDL per protein was found at a subfraction between LDL and HDL. The particle size of LDL in this fraction was smaller than that of LDL in the main LDL fraction, as assessed by electrophoresis. In addition, LDL oxidized by Cu2+ was also detectable with this method. We conclude that our method is sensitive and specific for MDA-LDL and might be useful for investigating MDA-LDL in the human circulation.
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
February 2001,
Nihon rinsho. Japanese journal of clinical medicine,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
March 1991,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
January 2003,
Journal of atherosclerosis and thrombosis,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
July 2017,
Clinical laboratory,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
January 2009,
Clinica chimica acta; international journal of clinical chemistry,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
May 2017,
International heart journal,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
August 2014,
Clinical and investigative medicine. Medecine clinique et experimentale,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
July 2020,
Nutrients,
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
April 2024,
Medicina (Kaunas, Lithuania),
K Kotani, and
M Maekawa, and
T Kanno, and
A Kondo, and
N Toda, and
M Manabe
September 2002,
Rheumatology (Oxford, England),
