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The teratogenic potential of caffeine was examined in vitro by a whole embryo culture system (WECS) and an embryonic cell culture system (micromas teratogen assay: MTA) in the rat. In the WECS, hyperemia of the tail, and a reduction of the placental size was induced by caffeine at concentrations higher than 50 micrograms/ml; hypoplasia of the forelimb bud was induced at concentrations higher than 100 micrograms/ml; hematoma in the yolk sac and dysmorphogenesis of the fore- and hind-limb buds, prosencephalon and tail were induced by 200 micrograms/ml caffeine. In the MTA, even with 200 micrograms/ml caffeine, the toxicological parameters obtained by proliferation and differentiation assays of the midbrain and limb bud cells were almost the same as in the control. In conclusion, caffeine induced various morphological anomalies, but did not affect proliferation or differentiation of cells in these experimental systems.
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
January 2005,
Reproductive toxicology (Elmsford, N.Y.),
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
December 1998,
Anesthesiology,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
January 1996,
Teratogenesis, carcinogenesis, and mutagenesis,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
January 1992,
Acta anatomica,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
December 2001,
Ginekologia polska,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
August 2000,
Teratology,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
June 2010,
Toxicological sciences : an official journal of the Society of Toxicology,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
January 1986,
Acta anatomica,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
January 1995,
Biology of the neonate,
T Iwase, and
K Arishima, and
N Ohyama, and
K Inazawa, and
Y Iwase, and
Y Ikeda, and
M Shirai, and
M Yamamoto, and
H Somiya, and
Y Eguchi
October 1982,
Journal of embryology and experimental morphology,
