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We have developed a method allowing suitable morphological conservation combined with in situ hybridization, on hippocampal slices used in conventional electrophysiological studies. After a bath application of kainate (KA, 750 nM, 2 min 15 s), electrical stimulation of the mossy fibre zone evoked epileptiform activity for up to 2 h. In situ hybridization performed on these slices showed a marked increased in expression of the transcription factor Zif/268 over the pyramidal and the granule cells and the surrounding neuropils. Bath application of tetraethylammonium (TEA, 25 mM, 10 min) elicited long-term potentiation in CA1 lasting up to 4 h. This was associated with enhanced expression of Zif/268 which returned to control values after 2 h 30 min. These observations suggest that slice preparations are suitable for the study of the role of neuronal activity in the regulation of gene expression.
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 1979,
Acta neurobiologiae experimentalis,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 2009,
Neuroscience and behavioral physiology,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
April 1999,
Brain research,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 2008,
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 1997,
Hippocampus,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 1987,
Brain research bulletin,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
September 1997,
Proceedings of the National Academy of Sciences of the United States of America,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
December 1999,
Brain research,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
January 1988,
Neurobiology of aging,
P Chinestra, and
X Leinekugel, and
Y Ben Ari, and
H Pollard
February 1995,
Neuropharmacology,
