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Upon the establishment of an effective nitrogen-fixing symbiosis in amide-transporting plants the enzymatic activity and transcript levels of L-asparaginase are dramatically decreased. This decrease in L-asparaginase activity is essential for the correct functioning of the Rhizobium-legume symbiosis in lupin in which asparagine, synthesized from recently fixed nitrogen, is exported to aerial parts of the plant for use in growth and development. Concomitant with this decrease in L-asparaginase transcript a DNA-binding protein was detected in the nodules. This binding protein was not detectable in ineffective nodules, in nodules treated with nitrate, or in root tips, mature roots, developing flowers or developing seeds. The DNA-binding activity was shown to interact with a 59 bp sequence proximal to the transcription start site. Within this sequence a CTAAAAT direct repeat and a ACTGT/TGTCA incomplete inverted repeat were implicated in the binding of protein to the DNA by DNase I protection experiments. Competitive binding studies with synthesized binding sites were consistent with the CTAAAAT/TGTCA sequence pair proximal to the transcription start site having the highest affinity for the DNA-binding protein. We postulate that this DNA-binding protein is associated with repression of L-asparaginase gene expression in mature lupin root nodules.
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
October 1992,
Plant molecular biology,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
April 1981,
Archives of biochemistry and biophysics,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
July 1992,
The New phytologist,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
November 2002,
Functional & integrative genomics,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
December 1994,
Plant physiology,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
June 1994,
Plant physiology,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
January 1993,
Journal of applied toxicology : JAT,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
January 2021,
Frontiers in genetics,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
January 1990,
Oecologia,
E Vincze, and
J M Reeves, and
E Lamping, and
K J Farnden, and
P H Reynolds
January 1962,
Acta biochimica Polonica,
