Biomaterial Database

Species-specific identification of Sarcocystis and Toxoplasma by PCR amplification of small subunit ribosomal RNA gene fragments. 1994

A M Tenter, and K Luton, and A M Johnson

Institut für Parasitologie der Tierärztlichen Hochschule Hannover, Germany.

The small subunit (SSU) ribosomal RNA (rRNA) genes of four cyst-forming coccidia (Sarcocystis tenella, Sarcocystis arieticanis, Sarcocystis gigantea, Toxoplasma gondii) infecting sheep were analysed for unique target sequences which could be used as priming sites for species-specific polymerase chain reactions (PCR). A total of 11 putatively species-specific oligonucleotides were tested in combination with universal oligonucleotides designed for conserved regions of the SSU rRNA genes of eukaryotes. For each parasite species two of these oligonucleotide pairs were found to be ideal primers for species-specific amplification of SSU rRNA gene fragments. The specificity of the PCR assays was optimised by testing different PCR parameters (annealing temperature, magnesium ion concentration, number of amplification cycles) with genomic DNA preparations derived from S. tenella, S. arieticanis, S. gigantea, T. gondii and sheep. The new PCR assays are a powerful tool for species-specific differentiation of the three ovine Sarcocystis spp. and T. gondii.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012335	RNA, Ribosomal	The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)	Ribosomal RNA,15S RNA,RNA, 15S
D012522	Sarcocystis	A genus of protozoa found in reptiles, birds, and mammals, including humans. This heteroxenous parasite produces muscle cysts in intermediate hosts such as domestic herbivores (cattle, sheep, pigs) and rodents. Final hosts are predators such as dogs, cats, and man.	Sarcosporidia,Sarcocysti,Sarcosporidias
D012523	Sarcocystosis	Infection of the striated muscle of mammals by parasites of the genus SARCOCYSTIS. Disease symptoms such as vomiting, diarrhea, muscle weakness, and paralysis are produced by sarcocystin, a toxin produced by the organism.	Sarcosporidiosis,Sarcocystoses,Sarcosporidioses
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D012757	Sheep Diseases	Diseases of domestic and mountain sheep of the genus Ovis.	Ovine Diseases,Disease, Ovine,Disease, Sheep,Diseases, Ovine,Diseases, Sheep,Ovine Disease,Sheep Disease