Isoelectric focusing of proteins in ultra-thin polyacrylamide gels.
1994
J M Walker
Division of Biosciences, University of Hertfordshire, Hatfield, UK.
UI
MeSH Term
Description
Entries
D007525
Isoelectric Focusing
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Electrofocusing,Focusing, Isoelectric
D007526
Isoelectric Point
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.