Biomaterial Database

Rapid assay of dinitrophenyl derivative of taurine by high-performance liquid chromatography. 1994

B Polanuer, and S Ivanov, and A Sholin

Institute for Genetics of Industrial Microorganisms, Moscow, Russian Federation.

A rapid and simple method for the determination of taurine (2-aminoethanesulphonic acid) in complex samples is described. It is based on the HPLC separation of the dinitrophenyl (DNP) derivative of taurine. The reaction conditions are selected to allow complete derivatization of taurine within 15 min. DNP-taurine samples are stable for at least 3 days. DNP-taurine was separated by reversed-phase liquid chromatography within 12 s. The recovery of taurine was 102 +/- 3% (S.D. = 2.5%, n = 6) and the detection limit was 10 pmol for taurine (signal-to-noise ratio of 10). The method was applied to the determination of taurine levels in different samples including marine products, infant formulas and fermentation media of different bacterial species.

UI	MeSH Term	Description	Entries
D007223	Infant	A child between 1 and 23 months of age.	Infants
D007225	Infant Food	Food processed and manufactured for the nutritional health of children in their first year of life.	Food, Infant,Foods, Infant,Infant Foods
D008460	Meat	The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game.	Meats
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004140	Dinitrophenols	Organic compounds that contain two nitro groups attached to a phenol.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001419	Bacteria	One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.	Eubacteria
D013654	Taurine	A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids.	Taufon,Tauphon,Taurine Hydrochloride,Taurine Zinc Salt (2:1),Taurine, Monopotassium Salt
D014413	Tuna	Common name for various species of large, vigorous ocean fishes in the family Scombridae.	Tunas