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High-performance liquid chromatographic determination of intrathecally administered [D-Ala2-D-Leu5]-enkephalin concentrations in canine cerebrospinal fluid. 1994

S Cox, and R Harvey, and D Frazier
Department of Environmental Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.

A rapid and useful method for high-performance liquid chromatographic analysis of exogenous [D-Ala2-D-Leu5]-enkephalin (DADLE) in cerebrospinal fluid (CSF) is described. CSF (0.5 ml) samples were filtered using a 0.22-micron Co-Star filter. Chromatography was performed on a mu Bondapak C18 column using a mobile phase of A, 0.05 M sodium phosphate (monobasic, pH 6.0) and B, 60% acetonitrile in 0.05 M sodium phosphate (pH 6.0) with a flow-rate of 1 ml/min. Absorbance at 210 nm was measured. The procedure produced a linear curve for the concentration range 1-10 micrograms/ml. The development of the assay produced rapid, repeatable and accurate results for CSF analysis of DADLE at concentrations achieved with therapeutic administration of the peptide. This method could also be used in the future for analysis of compounds like DADLE.

UI MeSH Term Description Entries
D011786 Quality Control A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed) Control, Quality,Controls, Quality,Quality Controls
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D016308 Enkephalin, Leucine-2-Alanine A delta-selective opioid (ANALGESICS, OPIOID). It can cause transient depression of mean arterial blood pressure and heart rate. 2-Alanyl-Leucine Enkephalin,5-Leucine-2-Alanine Enkephalin,D-Ala(2)-D-Leu(5)-Enkephalin,Enkephalin-Leu,Ala(2),Leucine Enkephalin-2-Alanine,BW-180C,DADLE,Leu-Enkephalin-2-Ala,2 Alanyl Leucine Enkephalin,5 Leucine 2 Alanine Enkephalin,BW 180C,BW180C,Enkephalin, 2-Alanyl-Leucine,Enkephalin, 5-Leucine-2-Alanine,Enkephalin, Leucine 2 Alanine,Enkephalin-2-Alanine, Leucine,Leu Enkephalin 2 Ala,Leucine Enkephalin 2 Alanine,Leucine-2-Alanine Enkephalin

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