Biomaterial Database

Development of gene probes of Dichelobacter nodosus for differentiating strains causing virulent, intermediate or benign ovine footrot. 1994

D Liu

Department of Agriculture, Pastoral and Veterinary Institute, Hamilton, Victoria, Australia.

Seven Dichelobacter nodosus genomic DNA clones including six specific for virulent and one for benign strains were identified. A collection of 96 footrot isolates, which in turn comprised 27 virulent isolates showing elastase activity at 7 days, 25 high intermediate isolates with elastase activity at 14 days, 24 low intermediate isolates with elastase activity at 21-28 days and 20 benign isolates with no elastase activity at up to 28 days, were used to assess these clones. Of the six virulent specific clones, five (pV238-83, pV405-239, pV470-65, pV470-145 and pV470-178) reacted with 27 virulent isolates, and 12 of 25 high intermediate isolates, but none of 24 low intermediate isolates and 20 benign isolates in dot blot hybridization. The other virulent-specific clone (pV470-13) recognized all 27 virulent and 25 intermediate isolates, and 22 of 24 low intermediate isolates and three of 20 benign isolates in dot blot hybridization. By contrast, the benign-specific clone (pB645-335) detected all 20 benign isolates and 24 low intermediate isolates, and also 13 of 25 high intermediate isolates, but none of 27 virulent isolates in dot blot hybridization. Southern hybridization analysis indicated that whereas clones pV238-83, pV405-239 and pV470-178 bound a Sau3A band of 0.5 kb, clones pV470-65 and pV470-145 recognized two Sau3A bands of 0.7 and 0.5 kb in virulent strains of serogroups A to I. However, clone pV470-13 detected a Sau3A band pattern in virulent strains different from those recognized by the other five virulent specific-clones. Besides showing a distinct Sau3A band pattern in intermediate strains, pV470-13 also reacted with three benign strains that showed binding with it in dot blot hybridization. The benign-specific clone pV645-335 detected a Sau3A band of 0.5 kb in both intermediate and benign strains of serogroups A to I. Thus the combination of pV470-13 and pB645-335, or any other virulent-specific clone, would clearly differentiate among D. nodosus strains causing virulent, intermediate or benign footrot.

UI	MeSH Term	Description	Entries
D009693	Nucleic Acid Hybridization	Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)	Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D005535	Foot Rot	A disease of the horny parts and of the adjacent soft structures of the feet of cattle, swine, and sheep. It is usually caused by Corynebacterium pyogenes or Bacteroides nodosus (see DICHELOBACTER NODOSUS). It is also known as interdigital necrobacillosis. (From Black's Veterinary Dictionary, 18th ed)	Foot Rots,Rot, Foot,Rots, Foot
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001439	Bacteroides	A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
D001442	Bacteroides Infections	Infections with bacteria of the genus BACTEROIDES.	Infections, Bacteroides,Bacteroides Infection,Infection, Bacteroides
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D012757	Sheep Diseases	Diseases of domestic and mountain sheep of the genus Ovis.	Ovine Diseases,Disease, Ovine,Disease, Sheep,Diseases, Ovine,Diseases, Sheep,Ovine Disease,Sheep Disease
D014774	Virulence	The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.	Pathogenicity
D015342	DNA Probes	Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.	Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal