-transparent.png){kind=logo}
The iron-molybdenum cofactor (FeMo-co) of nitrogenase is a unique molybdenum-containing prosthetic group that has been proposed to form an integral part of the active site of dinitrogenase. In Klebsiella pneumoniae, at least six nif (nitrogen fixation) gene products are required for the biosynthesis of FeMo-co, including NIFB, NIFNE, NIFH, NIFQ, and NIFV. An in vitro system for the synthesis of FeMo-co, which requires MgATP, molybdate, homocitrate, and at least the products of nifN, E, B, and H, has provided an enzymatic assay for the purification of many of the gene products required for FeMo-co biosynthesis. Although the structure of the cofactor has been solved recently, much about the biosynthetic pathway remains unknown. This article reviews what is known about the various components required for FeMo-co biosynthesis.
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
January 2008,
Annual review of microbiology,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
October 1988,
BioFactors (Oxford, England),
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
May 2013,
The Journal of biological chemistry,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
September 1984,
Journal of bacteriology,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
January 2002,
Metal ions in biological systems,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
October 1987,
The Journal of biological chemistry,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
May 1999,
The Journal of biological chemistry,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
August 1977,
Proceedings of the National Academy of Sciences of the United States of America,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
July 1992,
Proceedings of the National Academy of Sciences of the United States of America,
R M Allen, and
R Chatterjee, and
M S Madden, and
P W Ludden, and
V K Shah
February 2004,
Chemical reviews,
