-transparent.png){kind=logo}
We have recently shown that connexin 43 (Cx43), a major gap junction protein in osteoblasts, is expressed with an increase in cell density (CHIBA, H. et al. (1993). Cell Struc. Funct., 18: 419-426). In the present study, we examined what kinds of hormones and cytokines regulate the gap junction protein in osteoblastic cells, using a human osteoblastic cell line (SV-HFO) after reaching a confluent density to avoid influence of cell proliferation. Either retinoic acid (RA) or transforming growth factor-beta 1 (TGF-beta 1) induced the Cx43 expression of SV-HFO cells, as revealed by Northern blot analysis and immunocytochemistry. These modulators also increased gap junctional intercellular communication, in terms of the extent of dye transfer. On the other hand, 1 alpha, 25-dihydroxyvitamin D3 did not influence the Cx43 expression and gap junctional intercellular communication of the cells. These results suggest that RA and TGF-beta might maintain bone tissue as an organized tissue in vivo by increasing intercellular communication of osteoblastic cells.
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
September 1992,
Molecular endocrinology (Baltimore, Md.),
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
December 2006,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
May 2000,
American journal of obstetrics and gynecology,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
June 2021,
Biotechnology and applied biochemistry,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
July 2009,
American journal of physiology. Heart and circulatory physiology,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
August 1998,
Molecular biology of the cell,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
April 1998,
The Journal of biological chemistry,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
July 1995,
The Journal of cell biology,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
June 2007,
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban,
H Chiba, and
N Sawada, and
M Oyamada, and
T Kojima, and
K Iba, and
S Ishii, and
M Mori
January 2002,
The European journal of neuroscience,
