Biomaterial Database

[Detection of Plasmodium falciparum by polymerase chain reaction (PCR)]. 1994

B Zhan, and L Zhang, and J Wang, and X Feng

Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.

A pair of primers were designed and synthesized based on the DNA fragment coded erythrocyte-binding antigen (EBA-175) of Plasmodium falciparum (P.f.) and polymerase chain reaction (PCR) was performed to detect P.f. cultured in vitro. The amplified products were analysed by agarose gel electrophoresis and a specific 492 bp DNA band was showed in P.f. infected blood sample, but no band showed in P. vivax, P. cynomolgi, P. yoelii and P. berghei infected blood samples, nor in normal one. The parasite densities detected by this method could be as minimal as 10 parasites/20 microliters blood. The PCR technique showed high sensitivity and specificity.

UI	MeSH Term	Description	Entries
D010963	Plasmodium falciparum	A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.	Plasmodium falciparums,falciparums, Plasmodium
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000953	Antigens, Protozoan	Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.	Protozoan Antigens
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D015800	Protozoan Proteins	Proteins found in any species of protozoan.	Proteins, Protozoan
D016054	DNA, Protozoan	Deoxyribonucleic acid that makes up the genetic material of protozoa.	Protozoan DNA
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016778	Malaria, Falciparum	Malaria caused by PLASMODIUM FALCIPARUM. This is the severest form of malaria and is associated with the highest levels of parasites in the blood. This disease is characterized by irregularly recurring febrile paroxysms that in extreme cases occur with acute cerebral, renal, or gastrointestinal manifestations.	Plasmodium falciparum Malaria,Malaria, Plasmodium falciparum