BIOMAT Database Logo BIOMAT Database Logo

Agonist-induced up-regulation of platelet-activating factor receptor messenger RNA in human monocytes. 1994

H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Department of Thoracic Medicine, National Heart and Lung Institute, London, UK.

Platelet-activating factor (PAF) is a potent inflammatory mediator and it actions are mediated via specific cell surface receptors which are coupled to G-proteins. PAF stimulates several functions in monocytes and may modulate the expression of its own receptor. To investigate the possible modulation of PAF receptor mRNA expression Northern blot analysis of total RNA from human monocytes was performed using the cDNA of human leukocyte PAF receptor as a probe. Following the addition of 100 nM PAF, there was a 2.0-fold increase in PAF receptor mRNA at 60 minutes after the stimulation, which was inhibited by pretreatment with the PAF receptor antagonist WEB 2086. This increase returned to control level at 120 and 180 min. The increase of PAF receptor mRNA was statistically significant for 10 nM to 1 microM of PAF, while 100 nM of lysoPAF did not increase PAF receptor mRNA levels. These results suggest that PAF receptor expression can be regulated by PAF itself at the transcriptional level.

UI MeSH Term Description Entries
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D010972 Platelet Activating Factor A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION. AGEPC,Acetyl Glyceryl Ether Phosphorylcholine,PAF-Acether,Phosphorylcholine, Acetyl Glyceryl Ether,1-Alkyl-2-acetyl-sn-glycerophosphocholine,Platelet Aggregating Factor,Platelet Aggregation Enhancing Factor,Platelet-Activating Substance,Thrombocyte Aggregating Activity,1 Alkyl 2 acetyl sn glycerophosphocholine,Aggregating Factor, Platelet,Factor, Platelet Activating,PAF Acether,Platelet Activating Substance
D010980 Platelet Membrane Glycoproteins Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors. PM-GP,Platelet Glycoprotein,Platelet Membrane Glycoprotein,PM-GPs,Platelet Glycoproteins,Glycoprotein, Platelet,Glycoprotein, Platelet Membrane,Glycoproteins, Platelet,Glycoproteins, Platelet Membrane,Membrane Glycoprotein, Platelet,Membrane Glycoproteins, Platelet,PM GP
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001381 Azepines Seven membered heterocyclic rings containing a NITROGEN atom. Hexamethyleneimines
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014230 Triazoles Heterocyclic compounds containing a five-membered ring with two carbon atoms and three nitrogen atoms with the molecular formula C2H3N3. Triazole

Related Publications

H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes.
October 2007, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
IFN-gamma up-regulates platelet-activating factor receptor gene expression in human monocytes.
May 1994, Journal of immunology (Baltimore, Md. : 1950),
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Inverse agonist-induced signaling and down-regulation of the platelet-activating factor receptor.
October 2007, Cellular signalling,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Localization of platelet-activating factor receptor messenger RNA in the rat eye.
November 1997, Investigative ophthalmology & visual science,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Agonist-induced down-regulation of platelet-activating factor receptor gene expression in U937 cells.
August 1994, The Biochemical journal,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Regulation of platelet-activating factor synthesis in human monocytes by dipalmitoyl phosphatidylcholine.
July 2003, Journal of leukocyte biology,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Synthesis of platelet-activating factor by human monocytes stimulated by platelet-activating factor.
March 1991, The Journal of allergy and clinical immunology,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Structural and functional requirements for agonist-induced internalization of the human platelet-activating factor receptor.
August 1997, The Journal of biological chemistry,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Platelet-activating factor stimulates receptor-mediated formation of reactive oxygen intermediates in human monocytes.
December 1991, Lipids,
H Shirasaki, and I M Adcock, and O J Kwon, and M Nishikawa, and J C Mak, and P J Barnes
Priming of monocytes with interferon-gamma is associated with up-regulation of expression of the receptor for platelet-activating factor.
November 1994, Annals of the New York Academy of Sciences,
Copied contents to your clipboard!