Biomaterial Database

Differentiation of infectious pancreatic necrosis virus isolates by polymerase chain reaction. 1994

K M Shankar, and K L Roy, and T Yamamoto

Department of Microbiology, University of Alberta, Edmonton, Canada.

Infectious pancreatic necrosis virus (IPNV) from lake trout of Cornwall lake (LT-IPNV), from trout of Jasper (Ja-IPNV) and Arctic char of Northwest Territories (AC-IPNV) are the three isolates recorded from western Canada. Two segments (nt 453-674 and nt 1197-1503) from VP2 coding region of RNA of the isolates were amplified by polymerase chain reaction (PCR) for differentiation. Primers for cDNA synthesis and amplification by PCR were chosen from VP2 coding region of RNA of Ja-IPNV. The segment of 453-674 could be amplified in all the three isolates at annealing temperature from 50 to 60 degrees C. However the segment nt 1197-1503 could be amplified only from Jasper and not from AC or LT-INV at primer annealing temperature ranging from 50 degrees-60 degrees C. PCR product could be obtained from the latter two isolates only at Primer annealing temperature of 45 degrees C. This difference in annealing temperature in PCR amplification between the isolates could be used for differentiating the isolates at genome level.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003937	Diagnosis, Differential	Determination of which one of two or more diseases or conditions a patient is suffering from by systematically comparing and contrasting results of diagnostic measures.	Diagnoses, Differential,Differential Diagnoses,Differential Diagnosis
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D018061	Infectious pancreatic necrosis virus	The type species of AQUABIRNAVIRUS, causing infectious pancreatic necrosis in salmonid fish and other freshwater and marine animals including mollusks.	IPNV,Pancreatic Necrosis Virus, Infectious

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