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Two ELISA assays were developed to test the reactivity of soluble sHLAs with anti-HLA class I mAbs of IgG or IgM isotype. A panel of 40 different alleles of sHLA antigens was produced using 57 lymphoblastoid B-cell lines, which had been generated from class-I-phenotyped PBLs. Using 14 mAbs, the expression of 13 different sHLA antigens (sHLA-A2, -A3, -A11, -A24, -A29, -B7, -B8, -B13, -B14, -B27, -B44, -B57, and -B58) by 43 different cell lines was confirmed. In addition, the expected absence of these alleles from the culture supernatant of 11 cell lines was confirmed. Cross-reactivities of mAbs observed by microlymphocytotoxicity assays were also detected by ELISA. The results of this extensive analysis confirmed previous results demonstrating that sHLA class I typing by ELISA correlated with HLA class I cell typing by microlymphocytotoxicity [1-3]. Furthermore, additional information about the fine specificities of two mAbs was obtained. An anti-B27/44 IgM mAb appeared to react only with sHLA-B44 but not with sHLA-B27; mAb CR11-351, previously reported to react with HLA-A2, 28, bound also to sHLA-A1, -3, -11, and -A24.
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
November 1989,
Blut,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
January 1991,
Vox sanguinis,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
May 1997,
Clinical and diagnostic laboratory immunology,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
January 1984,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
March 1990,
Scandinavian journal of immunology,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
September 1995,
Human immunology,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
January 1997,
Human immunology,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
October 1986,
Journal of clinical microbiology,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
July 1996,
American journal of kidney diseases : the official journal of the National Kidney Foundation,
C Pouletty, and
I Mercier, and
L Glanville, and
N Tomavo, and
L Igoudin, and
P Pouletty, and
R Buelow
October 1983,
The Indian journal of medical research,
