BIOMAT Database Logo BIOMAT Database Logo

Airway epithelial cell expression of interleukin-6 in transgenic mice. Uncoupling of airway inflammation and bronchial hyperreactivity. 1994

B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

We produced transgenic mice which overexpress human IL-6 in the airway epithelial cells. Transgenic mice develop a mononuclear cell infiltrate adjacent to large and mid-sized airways. Immunohistochemistry reveals these cells to be predominantly CD4+ cells, MHC class II+ cells, and B220+ cells. Transgenic mice and nontransgenic mice had similar baseline respiratory system resistance (0.47 +/- 0.06 vs 0.43 +/- 0.04 cmH2O/ml per s at 9 wk of age, P = NS and 0.45 +/- 0.07 vs 0.43 +/- 0.09 cmH2O/ml per s at 17 wk of age, P = NS). Transgenic mice, however, required a significantly higher log dose of methacholine to produce a 100% increase in respiratory system resistance as compared with non-transgenic littermates (1.34 +/- 0.24 vs 0.34 +/- 0.05 mg/ml, P < or = 0.01). We conclude that the expression of human IL-6 in the airways of transgenic mice results in a CD4+, MHC class II+, B220+ lymphocytic infiltrate surrounding large and mid-sized airways that does not alter basal respiratory resistance, but does diminish airway reactivity to methacholine. These findings demonstrate an uncoupling of IL-6-induced airway lymphocytic inflammation and airway hyperresponsiveness and suggest that some forms of airway inflammation may serve to restore altered airway physiology.

UI MeSH Term Description Entries
D007249 Inflammation A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function. Innate Inflammatory Response,Inflammations,Inflammatory Response, Innate,Innate Inflammatory Responses
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008808 Mice, Inbred CBA An inbred strain of mouse that is widely used in BIOMEDICAL RESEARCH. Mice, CBA,Mouse, CBA,Mouse, Inbred CBA,CBA Mice,CBA Mice, Inbred,CBA Mouse,CBA Mouse, Inbred,Inbred CBA Mice,Inbred CBA Mouse
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D000403 Airway Resistance Physiologically, the opposition to flow of air caused by the forces of friction. As a part of pulmonary function testing, it is the ratio of driving pressure to the rate of air flow. Airway Resistances,Resistance, Airway,Resistances, Airway
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015850 Interleukin-6 A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS. Hepatocyte-Stimulating Factor,Hybridoma Growth Factor,IL-6,MGI-2,Myeloid Differentiation-Inducing Protein,Plasmacytoma Growth Factor,B Cell Stimulatory Factor-2,B-Cell Differentiation Factor,B-Cell Differentiation Factor-2,B-Cell Stimulatory Factor 2,B-Cell Stimulatory Factor-2,BSF-2,Differentiation Factor, B-Cell,Differentiation Factor-2, B-Cell,IFN-beta 2,IL6,Interferon beta-2,B Cell Differentiation Factor,B Cell Differentiation Factor 2,B Cell Stimulatory Factor 2,Differentiation Factor 2, B Cell,Differentiation Factor, B Cell,Differentiation-Inducing Protein, Myeloid,Growth Factor, Hybridoma,Growth Factor, Plasmacytoma,Hepatocyte Stimulating Factor,Interferon beta 2,Interleukin 6,Myeloid Differentiation Inducing Protein,beta-2, Interferon
D016210 Methacholine Chloride A quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1116) Methacholine,2-(Acetyloxy)-N,N,N-trimethyl-1-propanaminium Chloride,Acetyl-2-methylcholine Chloride,Acetyl-beta-methacholine Chloride,Acetyl-beta-methylcholine,Mecholine,Mecholyl,Provocholine,Provokit,Acetyl 2 methylcholine Chloride,Acetyl beta methacholine Chloride,Acetyl beta methylcholine,Chloride, Methacholine
D016535 Bronchial Hyperreactivity Tendency of the smooth muscle of the tracheobronchial tree to contract more intensely in response to a given stimulus than it does in the response seen in normal individuals. This condition is present in virtually all symptomatic patients with asthma. The most prominent manifestation of this smooth muscle contraction is a decrease in airway caliber that can be readily measured in the pulmonary function laboratory. Bronchial Hyperreactivities,Hyperreactivities, Bronchial,Hyperreactivity, Bronchial

Related Publications

B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Evaluation of airway hyperreactivity in interleukin-5 transgenic mice.
January 1995, International archives of allergy and immunology,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Expression of interleukin 9 in the lungs of transgenic mice causes airway inflammation, mast cell hyperplasia, and bronchial hyperresponsiveness.
October 1998, The Journal of experimental medicine,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Interleukin-5 and eosinophils induce airway damage and bronchial hyperreactivity during allergic airway inflammation in BALB/c mice.
June 1997, Immunology and cell biology,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Interferon-beta prevents antigen-induced bronchial inflammation and airway hyperreactivity in mice.
July 1997, Pharmacology,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Abrogation of bronchial eosinophilic inflammation and airway hyperreactivity in signal transducers and activators of transcription (STAT)6-deficient mice.
May 1998, The Journal of experimental medicine,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Bronchial epithelial cell-cytokine interactions in airway inflammation.
June 1995, Journal of investigative medicine : the official publication of the American Federation for Clinical Research,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
[Mechanisms of bronchial hyperreactivity: role of airway inflammation and atopy].
January 1994, Revue des maladies respiratoires,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Airway inflammation and remodeling in asthma. Lessons from interleukin 11 and interleukin 13 transgenic mice.
November 2001, American journal of respiratory and critical care medicine,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
Conditionally induced RAGE expression by proximal airway epithelial cells in transgenic mice causes lung inflammation.
October 2014, Respiratory research,
B F DiCosmo, and G P Geba, and D Picarella, and J A Elias, and J A Rankin, and B R Stripp, and J A Whitsett, and R A Flavell
IL-17RA Signaling in Airway Inflammation and Bronchial Hyperreactivity in Allergic Asthma.
December 2015, American journal of respiratory cell and molecular biology,
Copied contents to your clipboard!