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OBJECTIVE To investigate: (1) whether Helicobacter pylori directly induces interleukin-8 (IL-8) message expression and protein secretion in established gastric epithelial cell lines; and (2) if CagA/cytotoxin positive and negative strains of H pylori differ in their ability to induce epithelial IL-8. METHODS Gastric epithelial cell lines were co-cultured with H pylori NCTC 11637 and 10 clinical isolates (four cytotoxic, six non-cytotoxic) and secreted IL-8 was measured by enzyme linked immunosorbent assay (ELISA). Specific induction of gastric epithelial IL-8 mRNA was examined by reverse transcription and polymerase chain reaction (RT-PCR) amplification. RESULTS H pylori (NCTC 11637) induced IL-8 secretion from three gastric epithelial cell lines (KATO-3, ST42, AGS) but not from MKN 45 (gastric) or intestinal (SW480, HT29) cell lines. H mustelae did not stimulate IL-8 secretion from KATO-3, ST42, and AGS cells. H pylori induced IL-8 secretion was reduced by heat killing, sonication, freeze thawing or formalin fixation of the bacteria. CagA/cytotoxin positive strains of H pylori induced significantly higher IL-8 secretion than CagA/cytotoxin negative strains in the three positive gastric epithelial cell lines (KATO-3, ST42: p < 0.01; AGS: p < 0.02). A significant increase (p < 0.01) in the expression of IL-8 mRNA relative to G3PDH mRNA was observed in KATO-3 cells after three hours of co-culture with CagA/cytotoxin positive strains. CONCLUSIONS H pylori directly increases gastric epithelial IL-8 mRNA expression and IL-8 protein secretion in a strain specific manner. Induction of epithelial IL-8 by CagA/cytotoxin positive strains is likely to result in neutrophil chemotaxis and activation and thus mucosal damage. These observations on epithelial IL-8 may explain the association between CagA/cytotoxin positive strains and gastroduodenal disease.
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
December 2016,
Gastroenterology and hepatology from bed to bench,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
January 2006,
Cellular microbiology,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
May 1995,
Infection and immunity,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
June 1997,
Journal of the National Cancer Institute,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
February 2001,
Cancer research,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
May 1995,
Infection and immunity,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
August 2001,
Zhonghua nei ke za zhi,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
August 2002,
Scandinavian journal of gastroenterology,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
October 1995,
Journal of clinical pathology,
J E Crabtree, and
S M Farmery, and
I J Lindley, and
N Figura, and
P Peichl, and
D S Tompkins
February 2016,
The Journal of infectious diseases,
