Biomaterial Database

5-Hydroxytryptamine-2 and -4 receptors located on bovine isolated mesenteric lymphatics. 1994

H Miyahara, and Y Kawai, and T Ohhashi

1st Department of Physiology, Shinshu University School of Medicine, Matsumoto, Japan.

Effects of 5-hydroxytryptamine (5-HT) agonists were investigated in the bovine isolated mesenteric lymphatics, and then the 5-HT receptor subtypes in the responses were pharmacologically classified by use of several 5-HT antagonists. Addition of 5-HT and that of alpha-methyl-5-hydroxytryptamine (alpha-methyl-5-HT) both produced concentration-dependent contractions in the presence of 3 x 10(-6) M [(1 alpha H, 5 alpha H)-8-methyl-8-azabicyclo(3.2.1)oct-3 alpha-yl]1H-indole-3-carboxylate hydrochloride, whereas 5-carboxamidotryptamine (5-CT) and 2-methyl-5-hydroxytryptamine (2-methyl-5-HT) caused no, or only slight, contraction in the lymphatics. Pretreatment with ketanserin caused a significant shift to the right in the concentration-response curve for 5-HT or alpha-methyl-5-hydroxytryptamine. Schild plot analyses showed that the pA2 values for ketanserin against 5-HT and alpha-methyl-5-hydroxytryptamine were 8.75 and 8.68, respectively. In contrast, 5-CT, 5-HT and metoclopramide caused concentration-dependent relaxations in the presence of 10(-6) M ketanserin in the lymph vessels after contraction with prostaglandin F2 alpha. 2-Methyl-5-HT up to 10(-4) M did not produce any relaxation. High concentrations of [(1 alpha H, 5 alpha H)-8-methyl-8-azabicyclo(3.2.1)oct-3 alpha-y1]1H-indole-3-carboxylate hydrochloride induced a significant inhibition of the 5-CT- and 5-HT-induced relaxation, but methiothepin and methysergide up to 10(-6) M and mechanical rubbing of the endothelium had no significant effect on the relaxation.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008208	Lymphatic System	A system of organs and tissues that process and transport immune cells and LYMPH.	Lymphatic Systems
D008643	Mesentery	A layer of the peritoneum which attaches the abdominal viscera to the ABDOMINAL WALL and conveys their blood vessels and nerves.	Mesenteries
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D011985	Receptors, Serotonin	Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action.	5-HT Receptor,5-HT Receptors,5-Hydroxytryptamine Receptor,5-Hydroxytryptamine Receptors,Receptors, Tryptamine,Serotonin Receptor,Serotonin Receptors,Tryptamine Receptor,Tryptamine Receptors,Receptors, 5-HT,Receptors, 5-Hydroxytryptamine,5 HT Receptor,5 HT Receptors,5 Hydroxytryptamine Receptor,5 Hydroxytryptamine Receptors,Receptor, 5-HT,Receptor, 5-Hydroxytryptamine,Receptor, Serotonin,Receptor, Tryptamine,Receptors, 5 HT,Receptors, 5 Hydroxytryptamine
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017366	Serotonin Receptor Agonists	Endogenous compounds and drugs that bind to and activate SEROTONIN RECEPTORS. Many serotonin receptor agonists are used as ANTIDEPRESSANTS; ANXIOLYTICS; and in the treatment of MIGRAINE DISORDERS.	5-HT Agonists,5-Hydroxytryptamine Agonists,Serotonin Agonists,5-HT Agonist,5-Hydroxytrytamine Agonist,Receptor Agonists, Serotonin,Serotonergic Agonist,Serotonergic Agonists,Serotonin Agonist,Serotonin Receptor Agonist,5 HT Agonist,5 HT Agonists,5 Hydroxytryptamine Agonists,5 Hydroxytrytamine Agonist,Agonist, 5-HT,Agonist, 5-Hydroxytrytamine,Agonist, Serotonergic,Agonist, Serotonin,Agonist, Serotonin Receptor,Agonists, 5-HT,Agonists, 5-Hydroxytryptamine,Agonists, Serotonergic,Agonists, Serotonin,Agonists, Serotonin Receptor,Receptor Agonist, Serotonin
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In