Biomaterial Database

Enhancement of concanavalin A-induced proliferation of spleno-lymphocytes by low-dose-irradiated macrophages. 1994

Y Ibuki, and R Goto

Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Japan.

The effect of whole-body irradiation with low doses of gamma-rays was investigated for the Con A-induced proliferation of splenocytes. Proliferation was enhanced by 0.02 Gy irradiation, but was inhibited by 0.2 Gy. The enhanced proliferation produced by low-dose irradiation also was studied using co-cultures of spleno-lymphocytes and peritoneal macrophages (M phi s) instead of spleno-M phi s. The proliferative response of spleno-lymphocytes was not affected by low doses of gamma-ray irradiation; whereas, the responses of unirradiated spleno-lymphocytes cultured with the peritoneal M phi s that had been irradiated at 0.02 and 0.04 Gy respectively were about 120 and 145% of the control. Proliferative enhancement was found when peritoneal M phi s collected 4 h after whole-body irradiation were used, but not with those collected at 1 and 12 h. No enhancement took place when in vitro irradiation was used. These results suggest that the enhancement in Con A-induced proliferation of splenocytes by low-dose irradiation was caused not by the activation of spleno-lymphocytes, but by activation of spleno-M phi s, and that the M phi s were activated indirectly.

UI	MeSH Term	Description	Entries
D008213	Lymphocyte Activation	Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.	Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D011829	Radiation Dosage	The amount of radiation energy that is deposited in a unit mass of material, such as tissues of plants or animal. In RADIOTHERAPY, radiation dosage is expressed in gray units (Gy). In RADIOLOGIC HEALTH, the dosage is expressed by the product of absorbed dose (Gy) and quality factor (a function of linear energy transfer), and is called radiation dose equivalent in sievert units (Sv).	Sievert Units,Dosage, Radiation,Gray Units,Gy Radiation,Sv Radiation Dose Equivalent,Dosages, Radiation,Radiation Dosages,Units, Gray,Units, Sievert
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D005720	Gamma Rays	Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source.	Gamma Wave,Gamma Radiation,Nuclear X-Rays,Radiation, Gamma,X-Rays, Nuclear,Gamma Radiations,Gamma Ray,Gamma Waves,Nuclear X Rays,Nuclear X-Ray,Ray, Gamma,Wave, Gamma,Waves, Gamma,X Rays, Nuclear,X-Ray, Nuclear
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013154	Spleen	An encapsulated lymphatic organ through which venous blood filters.
D014916	Whole-Body Irradiation	Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.	Radiation, Whole-Body,Total Body Irradiation,Irradiation, Total Body,Irradiation, Whole-Body,Whole-Body Radiation,Irradiation, Whole Body,Irradiations, Total Body,Irradiations, Whole-Body,Radiation, Whole Body,Radiations, Whole-Body,Total Body Irradiations,Whole Body Irradiation,Whole Body Radiation,Whole-Body Irradiations,Whole-Body Radiations
D017737	Macrophages, Peritoneal	Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.	Peritoneal Macrophages,Macrophage, Peritoneal,Peritoneal Macrophage
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus