Biomaterial Database

Measurement of intracellular pH in isolated human detrusor smooth muscle cells. 1994

C H Fry, and C R Gallegos, and B S Montgomery

Department of Physiology, United Medical School, London, United Kingdom.

Intracellular pH, pHi, was measured in isolated human detrusor smooth muscle cells by epifluorescence microscopy. The mean value was 7.07 +/- 0.19 when superfused with a CO2/HCO3- solution. Alteration of superfusate PCO2 also changed pHi whereas NaHCO3 changes were without effect, suggesting that the cell membrane is permeable to CO2 but impermeable to HCO3-. Nonbicarbonate H+ buffering power of the cell was calculated as 20.3 mequiv. x 1(-1) x pH unit-1. Addition of NH4Cl to the superfusate induced an alkalosis and, upon removal, a transient acidosis. The recovery from the acidosis allowed calculation of the acid extrusion rate from the cell. The rate was dependent on pHi and increased as the cell was more acid.

UI	MeSH Term	Description	Entries
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D001743	Urinary Bladder	A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.	Bladder,Bladder Detrusor Muscle,Detrusor Urinae,Bladder Detrusor Muscles,Bladder, Urinary,Detrusor Muscle, Bladder,Detrusor Muscles, Bladder
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000643	Ammonium Chloride	An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.	Sal Ammoniac,Ammoniac, Sal,Chloride, Ammonium