Biomaterial Database

Prepro-TRH-(160-169) potentiates gastric acid secretion stimulated by TRH microinjected into the dorsal motor nucleus of the vagus. 1994

H Yang, and Y Taché

CURE/Gastroenteric Biology Center, VA Wadsworth Medical Center, Los Angeles, CA 90073.

Prepro-TRH-(160-169) (Ps4), one of the predicted connecting peptides of pro-TRH, potentiates TRH-induced TSH release in vivo and in vitro. The influence of Ps4 on TRH in medullary nuclei-induced vagal stimulation of gastric acid secretion was studied in urethane-anesthetized rats with gastric cannula. Ps4 injected into the dorsal motor nucleus of the vagus (DMN) (200 ng) did not influence basal gastric secretion. Ps4 (100, 150 and 200 ng) co-injected with TRH (50 ng) into the DMN potentiated the acid response to TRH by 14%, 76% and 182% respectively while prepro-TRH-(178-199) (Ps5, 438 ng) had no effect. Ps4 (200 ng) co-injected with TRH into the nucleus ambiguus did not modify TRH (50 ng)-induced acid secretion. These results indicate that Ps4 potentiates TRH in the DMN-evoked gastric acid secretion through an action that is peptide and site specific.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008845	Microinjections	The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.	Microinjection
D009411	Nerve Endings	Branch-like terminations of NERVE FIBERS, sensory or motor NEURONS. Endings of sensory neurons are the beginnings of afferent pathway to the CENTRAL NERVOUS SYSTEM. Endings of motor neurons are the terminals of axons at the muscle cells. Nerve endings which release neurotransmitters are called PRESYNAPTIC TERMINALS.	Ending, Nerve,Endings, Nerve,Nerve Ending
D009412	Nerve Fibers	Slender processes of NEURONS, including the AXONS and their glial envelopes (MYELIN SHEATH). Nerve fibers conduct nerve impulses to and from the CENTRAL NERVOUS SYSTEM.	Cerebellar Mossy Fibers,Mossy Fibers, Cerebellar,Cerebellar Mossy Fiber,Mossy Fiber, Cerebellar,Nerve Fiber
D010275	Parasympathetic Nervous System	The craniosacral division of the autonomic nervous system. The cell bodies of the parasympathetic preganglionic fibers are in brain stem nuclei and in the sacral spinal cord. They synapse in cranial autonomic ganglia or in terminal ganglia near target organs. The parasympathetic nervous system generally acts to conserve resources and restore homeostasis, often with effects reciprocal to the sympathetic nervous system.	Nervous System, Parasympathetic,Nervous Systems, Parasympathetic,Parasympathetic Nervous Systems,System, Parasympathetic Nervous,Systems, Parasympathetic Nervous
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D011498	Protein Precursors		Precursors, Protein
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005744	Gastric Acid	Hydrochloric acid present in GASTRIC JUICE.	Hydrochloric Acid, Gastric,Acids, Gastric,Acids, Gastric Hydrochloric,Gastric Acids,Gastric Hydrochloric Acid,Gastric Hydrochloric Acids,Hydrochloric Acids, Gastric
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia