We explored the oxytocin-prostaglandin interactions during the rat estrous cycle. The experiments were done with uterine preparations isolated from rats in different stages of the sex cycle incubated 'in vitro' with oxytocin (O) (50 mU/ml). We found that the effect of O on prostaglandin (PG) synthesis was associated to the sex hormones, and varied during the estrous cycle. Indeed, during the estrogenic influence (i.e. at proestrus and estrus) O diminished the synthesis of PGE2 and with the highest estradiol concentration (i.e. during estrus) the hormone also augmented the synthesis of PGF2 alpha. During metestrus, no changes in PG synthesis after treatment were found. Likewise, during diestrus, when progesterone levels fall, O enhanced PGF2 alpha uterine synthesis. In this study an inhibitory action of O on the uterine production of 6-keto-PGF1 alpha at proestrus was also seen. The present results indicate that when estrogen concentration increases (during estrus) 6-keto-PGF1 alpha synthesis also increases. In summary, we have observed that sex hormones exert a modulating action on the influences of O on uterine PGs synthesis.