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We have developed monoclonal antibodies to most HLA specificities, making it possible for us to devise a simple, rapid, one-step microcytotoxicity test. The test is performed by adding 1 microliter of cells to 1 microliter of antibody-complement mixture predotted on the microtest tray. The reactions are read following a 1-hour incubation period (30 minutes in some instances). The analysis of reactions seen on testing 105 class I antibodies and 50 class II antibodies is shown. A comparison of typing by the standard NIH method and the new one-step procedure showed a > 96% concordance in the 500 T cells and 200 B cells we examined. Class I and class II typing could be performed using B cells, thus obviating the need to isolate both T and B cells for HLA typing.
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
January 2024,
HLA,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
May 1985,
Human immunology,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
June 2005,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
December 1998,
Bone marrow transplantation,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
January 1998,
Tissue antigens,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
January 2003,
Methods in molecular biology (Clifton, N.J.),
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
July 1986,
Nihon saikingaku zasshi. Japanese journal of bacteriology,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
September 1987,
Nihon saikingaku zasshi. Japanese journal of bacteriology,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
January 1984,
Immunogenetics,
J H Lee, and
M Lias, and
C T Deng, and
S Etessami, and
J Loon, and
M Chen, and
L Banh, and
N Conger, and
D Connors, and
A Manzo
November 1991,
PCR methods and applications,
