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A rapid and sensitive high-performance liquid chromatographic method for the analysis of the antiepileptic felbamate in human serum has been developed. Following protein precipitation with acetonitrile containing the internal standard alphenal, the drugs are extracted from serum using dichloromethane. The extraction efficiency averages 98% for felbamate. Chromatography is performed with use of reversed phase octyl (C8) column, monitored at 210 nm. The isocratic mobile phase is acetonitrile:acetate buffer, pH 5.3, flow rate 2.0 ml/min. Felbamate is resolved from other common antiepileptic drugs that may be co-administered. The assay is linear to 200 micrograms/ml in serum. The effect of mobile phase pH changes on the chromatographic characteristics of the antiepileptic drugs and some potential interferents is discussed.
T M Annesley, and
L T Clayton
June 1997,
Therapeutic drug monitoring,
T M Annesley, and
L T Clayton
February 1994,
Therapeutic drug monitoring,
T M Annesley, and
L T Clayton
January 2005,
Annales de biologie clinique,
T M Annesley, and
L T Clayton
January 1998,
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University,
T M Annesley, and
L T Clayton
August 1990,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
T M Annesley, and
L T Clayton
May 1983,
Journal of chromatography,
T M Annesley, and
L T Clayton
December 1982,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
T M Annesley, and
L T Clayton
January 1992,
Journal of chromatography,
T M Annesley, and
L T Clayton
March 1997,
Se pu = Chinese journal of chromatography,
T M Annesley, and
L T Clayton
November 1997,
Se pu = Chinese journal of chromatography,
