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A high performance liquid chromatographic assay was developed for determination of the plasma concentration of hexamethylene bisacetamide (HMBA) in rabbit. Plasma samples were vortex-mixed with 0.3 ml of methanol and centrifuged at 10000 r/min for 5 min. A 20 microliters aliquot of the supernatant was injected into the HPLC system. Analysis was performed on a Du Pont Zorbax C18 column (250 mm x 4.6 mm, 10 microns) with a precolumn (50 mm x 4.6 mm, 10 microns, YWG-C18). The eluent was methanol--water (30:70), with flow rate of 1.0 ml.min-1 and UV detection at 210 nm. The limit of detection was 1.0 microgram.ml-1 in plasma and the calibration curve was linear over the range 5-1000 micrograms.ml-1 (r = 0.9988). The HPLC assay was applied for studying the pharmacokinetics of HMBA in rabbit.
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
March 1987,
Journal of chromatography,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
January 1987,
Journal of chromatography,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
January 1985,
Anesthesia and analgesia,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
April 1984,
Anesthesia and analgesia,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
February 1991,
Journal of pharmaceutical sciences,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
July 1997,
Se pu = Chinese journal of chromatography,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
August 1982,
Journal of chromatography,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
October 1982,
Chemical & pharmaceutical bulletin,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
November 1982,
Journal of chromatography,
Y X Zhao, and
X Y He, and
M Jiang, and
J W Xie, and
T Q Xie, and
L Q Ren
November 1982,
Journal of chromatography,
