Biomaterial Database

A fluorimetric assay for the determination of anthranilic acid in biological materials. 1994

P Guidetti, and J L Walsh, and R Schwarcz

Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore 21228.

In the brain, anthranilic acid may serve as a bioprecursor of the endogenous excitotoxin quinolinic acid. Using a novel isolation procedure followed by HPLC and fluorimetric detection, we have developed an assay which is sufficiently sensitive to determine anthranilic acid in small (> or = 3 mg) samples of rat brain tissue (sensitivity limit: 50 fmol). Anthranilic acid was identified by its retention time in three chromatographic systems. The assay was applied to the measurement of anthranilic acid in rat serum (131 +/- 7 nM) and urine (9.9 +/- 118 nmol/mg creatinine) and in several organs which contained between 0.5 and 2 pmol anthranilic acid/mg protein. Only small differences in anthranilic acid content were found among 10 regions of the rat brain. Neuronal depletion induced by an intrastriatal excitotoxin injection resulted in an increase in anthranilic acid levels, suggesting a nonneuronal localization of the metabolite in the brain. This assay should provide an improved means for the investigation of the neurobiology of anthranilic acid.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D001923	Brain Chemistry	Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.	Chemistry, Brain,Brain Chemistries,Chemistries, Brain
D002138	Calibration	Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.	Calibrations
D003342	Corpus Striatum	Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.	Lenticular Nucleus,Lentiform Nucleus,Lentiform Nuclei,Nucleus Lentiformis,Lentiformis, Nucleus,Nuclei, Lentiform,Nucleus, Lenticular,Nucleus, Lentiform,Striatum, Corpus
D005470	Fluorometry	An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS.	Fluorimetry,Fluorometric Analysis,Analysis, Fluorometric
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D014781	Viscera	Any of the large interior organs in any one of the three great cavities of the body, especially in the abdomen.
D017207	Rats, Sprague-Dawley	A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.	Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats