-transparent.png){kind=logo}
Addition of periodate-oxidized ATP (oATP) to firefly luciferase-containing reaction mixtures enhanced light production when the reaction mixture contained > approximately 8 microM ATP. The time course of light production was changed from a flash pattern to a constant light output during incubations of < approximately 10 min. During longer incubation, firefly luciferase was inactivated in a concentration-dependent fashion by oATP. Firefly luciferase has two different time courses of light production that depend on ATP concentration (DeLuca and McElroy, Biochem. Biophys. Res. Commun., 123, 764, 1984). The enhancement of light production occurred only when higher ATP concentrations (> 8 microM) were used. There is little effect of oATP on firefly luciferase activity at low ATP concentrations (< 2 microM) which gave steady production of light. ATP did not antagonize the inactivation of firefly luciferase by oATP. When the oATP was chemically reduced with sodium borohydride (giving or ATP), there was no inactivation of firefly luciferase on incubation. When or ATP was used in a short incubation the enhancement of light production and time course change were the same as those observed with oATP. The corresponding AMP and adenosine compounds (o and or) were slightly inhibitory to firefly luciferase activity. ADP was without effect but both oADP and or ADP enhanced light production. Of these periodate-oxidized ADP, AMP, and adenosine derivatives only oADP inactivated firefly luciferase. The activating effect can be explained by a change in the conformation of the enzyme-product complex so that the product is released faster. In addition there is an inactivation of the enzyme by certain periodate-oxidized nucleotides during longer incubations.
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
July 1980,
Analytical biochemistry,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
August 1992,
Analytical biochemistry,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
February 1995,
The Biochemical journal,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
June 1982,
Photochemistry and photobiology,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
September 1984,
Biochemical and biophysical research communications,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
April 1975,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
March 2001,
Proteins,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
January 1991,
Journal of bioluminescence and chemiluminescence,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
May 2006,
Analytical biochemistry,
S R Ford, and
K D Chenault, and
M S Hall, and
S J Pangburn, and
F R Leach
January 1976,
Advances in enzymology and related areas of molecular biology,
