Biomaterial Database

ELISA for detecting dengue and Japanese encephalitis viral antigen in mosquitoes. 1994

R Sithiprasasna, and D Strickman, and B L Innis, and K J Linthicum

Department of Entomology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.

An indirect ELISA has been developed for detecting two viruses in triturated, experimentally infected mosquitoes: dengue (DEN) in Aedes aegypti (L.) and Ae. albopictus (Skuse) and Japanese encephalitis (JE) viral antigen in Culex tritaeniorhynchus Giles. DEN antigen from four strains of the virus, representing each of the four serotypes, and JE antigen were captured with a polyclonal anti-flavivirus IgG and detected with a monoclonal antibody (4G2) that reacts with all flaviviruses. Minimum viral titres detectable by the flavivirus antigen-capture ELISA, measured as multiples of the dose infecting 50% of mosquitoes (MID50), were 10(6.4), 10(4.5), 10(6.3), 10(6.9) and < or = 10(6.9) MID50/ml for DEN 1, DEN 2, DEN 3, DEN 4 and JE, respectively. Pools of up to 100 larvae or adults of Ae. aegypti and Ae. albopictus did not reduce the sensitivity of the ELISA to DEN 1, 2, 3 or 4. The results indicate that the antigen-capture ELISA could readily screen for DEN antigen in individual and pooled mosquitoes.

UI	MeSH Term	Description	Entries
D003465	Culex	A genus of mosquitoes (CULICIDAE) commonly found in tropical regions. Species of this genus are vectors for ST. LOUIS ENCEPHALITIS as well as many other diseases of man and domestic and wild animals.	Culices
D003716	Dengue Virus	A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known.	Breakbone Fever Virus,Breakbone Fever Viruses,Dengue Viruses,Fever Virus, Breakbone,Fever Viruses, Breakbone,Virus, Breakbone Fever,Virus, Dengue,Viruses, Breakbone Fever,Viruses, Dengue
D004664	Encephalitis Virus, Japanese	A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), which is the etiological agent of Japanese encephalitis found in Asia, southeast Asia, and the Indian subcontinent.	Japanese B Encephalitis Virus,Japanese Encephalitis Virus,Virus, Japanese Encephalitis
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000330	Aedes	A genus of mosquitoes (CULICIDAE) frequently found in tropical and subtropical regions. YELLOW FEVER and DENGUE are two of the diseases that can be transmitted by species of this genus.	Aede
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity